Morroniside ameliorates lipopolysaccharide-induced inflammatory damage in iris pigment epithelial cells through inhibition of TLR4/JAK2/STAT3 pathway  

作　　者：Wen-Jie Li Lin Liu Hong Lu 

机构地区：[1]The First Clinical Medical College,Lanzhou University,Lanzhou 730000,Gansu Province,China [2]Department of Ophthalmology,The First Hospital of Lanzhou University,Lanzhou 730000,Gansu Province,China [3]Department of Ophthalmology,Beijing Chao Yang Hospital,Capital Medical University,Beijing 100083,China

出　　处：《International Journal of Ophthalmology(English edition)》2023年第12期1928-1934,共7页国际眼科杂志（英文版）

基　　金：Supported by the Natural Science Foundation of Gansu Province(No.23JRRA0942);Innovation Fund for Colleges and Universities in Gansu Province(No.2021B-23).

摘　　要：AIM:To investigate the effect of morroniside(Mor)on lipopolysaccharide(LPS)-treated iris pigment epithelial cells(IPE).METHODS:IPE cells were induced by LPS and treated with Mor.Cell proliferation was detected by cell counting kit(CCK)-8,apoptosis was detected by flow cytometry,the levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-6,and IL-8 were measured by enzyme-linked immunosorbent assay(ELISA)kits,and the protein expression of TLR4,JAK2,p-JAK2,STAT3,and p-STAT3 was analyzed by Western blotting.In addition,overexpression of TLR4 and Mor treatment of LPS-stimulated IPE cells were also tested for the above indices.RESULTS:Mor effectively promoted the proliferation and inhibited the apoptosis of LPS-treated IPE cells.In addition,Mor significantly reduced the levels of TNF-α,IL-6,and IL-8 and significantly inhibited the expression of TLR4,p-JAK2,and p-STAT3 in LPS-treated IPE cells.The effect of Mor on LPS-treated IPE cells was markedly attenuated after overexpression of TLR4.CONCLUSION:These findings suggest that Mor may ameliorate LPS-induced inflammatory damage and apoptosis in IPE through inhibition of TLR4/JAK2/STAT3 pathway.

关 键 词：MORRONISIDE iris pigment epithelial cells INFLAMMATORY TLR4/JAK2/STAT3 pathway 

分 类 号：R774.1[医药卫生—眼科]