食用农产品中农药多残留高通量非靶向检测能力验证研究  

Proficiency Testing of High-throughput Non-targeted Detection of Pesticide Residues in Edible Agricultural Products

作　　者：雷思佳 谢瑜杰 余丽波 卜丹丹 霍思宇 陈辉[1] 陈冬东[1] 彭涛[1] LEI Sijia;XIE Yujie;YU Libo;BU Dandan;HUO Siyu;CHEN Hui;CHEN Dongdong;PENG Tao(Chinese Academy of Inspection and Quarantine,Beijing 100176,China;CAIQ(Beijing)Testing and Certification Co.,Ltd.,Beijing 100176,China;CAIQ(Beijing)Science and Technology Co.,Ltd.,Beijing 100176,China)

机构地区：[1]中国检验检疫科学研究院,北京100176 [2]中检科(北京)测试认证有限公司,北京100176 [3]检科院(北京)科学技术有限公司,北京100176

出　　处：《食品工业科技》2023年第24期270-278,共9页Science and Technology of Food Industry

基　　金：现场执法快检产品多维评价技术研究与认证应用(2022YFF1101004)。

摘　　要：为了解我国检验检测机构农药多残留高通量非靶向检测技术水平,提高食品安全风险监测能力,以有机苹果为原料制备空白和添加样品,采用F检验和SS≤0.3σ准则评估样品的均匀性,t检验和|x-y|≤0.3σ准则评估样品的稳定性,并用于能力验证的考核。针对现有评价技术未考虑高通量非靶向残留筛查的问题,提出一种新的评价方法,通过建立二维(XY)分类,从农药检出数量(维度X:定性)和可接受z比分数数量(维度Y:定量)进行考核,并通过定性满意率和定量满意率引入c值作为一级评价指标,假阳性率和假阴性率作为二级评价指标,综合评估实验室的检测能力。结果显示,本次能力验证共有20家实验室参加,定性满意率70%,定量满意率45%,综合满意率40%,对于c值为0的实验室,进一步通过二级指标评价,发现87.5%的实验室未出现假阳性和假阴性,评价方式合理。本研究将定性分析和定量分析法联合应用于高通量非靶向农药多残留检测能力验证结果评价,对优化其他能力评价方式提供了新思路。In order to understand the technical level of high-throughput non-targeted detection of pesticide residues in China's inspection and testing institutions and improve the ability of food safety risk monitoring.Blank and added samples were prepared with organic apples as raw materials.F test and SS≤0.3σ criterion were used to evaluate the uniformity of the samples.T test and |x-y|≤0.3σ criterion were used to evaluate the stability of the samples.In view of the fact that the existing evaluation technology did not consider the problem of high-throughput non-targeted detection,a new evaluation method was proposed.By establishing a two-dimensional(XY)classification,the number of pesticide detection(dimension X:qualitative)and the number of acceptable z-scores(dimension Y:quantitative)were evaluated.The c value was introduced as the primary evaluation index through qualitative satisfaction rate and quantitative satisfaction rate,while the false positive rate and false negative rate were used as the secondary evaluation index to comprehensively evaluate the detection ability of the laboratory.The results showed that a total of 20 laboratories participated in this proficiency testing,with a qualitative satisfaction rate of 70%,a quantitative satisfaction rate of 45%,and a comprehensive satisfaction rate of 40%.For laboratories with a c value of 0,further evaluation by secondary indicators showed that 87.5% of the laboratories did not show false positives and false negatives.In this study,qualitative analysis and quantitative analysis were combined to evaluate the results of high-throughput non-targeted detection of pesticide residues,which provided new ideas for the optimization of other ability evaluation methods.

关 键 词：农药多残留 高通量 非靶向检测 能力验证 定性定量评价 

分 类 号：TS207.3[轻工技术与工程—食品科学]