红托竹荪菌托多糖提取工艺优化及其泡腾片的制备  

Extraction Optimization and Preparation of Effervescent Tablets of Polysaccharides from Volva of Dictyophora rubrovalvata

作　　者：李莹[1] 周克春 石芳 林栋[1] LI Ying;ZHOU Kechun;SHI Fang;LIN Dong(Department of Food Science and Engineering,Guiyang University,Guiyang 550005,Guizhou,China)

机构地区：[1]贵阳学院食品科学与工程学院,贵州贵阳550005

出　　处：《食品研究与开发》2023年第23期123-129,共7页Food Research and Development

基　　金：贵州省教育厅高等学校科学研究项目(青年项目)(黔教技〔2022〕301号);2021年度贵阳学院学科团队建设项目([2021-xk15]007);贵州省生物制药工程研究中心建设项目(黔教合KY字[2019]051)。

摘　　要：为提高红托竹荪菌托中多糖的提取率,制备红托竹荪多糖泡腾片。该文考察提取时间、提取温度、料液比、提取功率对多糖得率的影响。在单因素试验的基础上,采用响应面法Box-Behnken设计,优化红托竹荪菌托多糖的提取工艺,并以提取出的多糖为原料,采用酸碱分开制粒混合压片的方法制备泡腾片。结果表明:红托竹荪菌托多糖提取的最佳工艺参数为提取时间51 min、提取温度60℃、料液比1∶48(g/mL)、提取功率400 W,测得实际多糖得率为13.86%。竹荪菌托多糖泡腾片最佳配比组成为竹荪菌托多糖2%、泡腾崩解剂总量45%、酸碱比(柠檬酸∶碳酸氢钠)1.5∶1、黏合剂(3%PVP水溶液)2%、润滑剂(PEG 6000)2%、甜味剂(阿斯巴甜)1%。所得泡腾片质量稳定,重现性好,符合2020版《中国药典》规定。To improve the yield of polysaccharides from the volva of Dictyophora rubrovalvata,this study prepared D.rubrovalvata polysaccharide effervescent tablets and investigated the effects of extraction time,extraction temperature,solid-to-liquid ratio,and extraction power on the yield of polysaccharides.Extraction conditions of polysaccharides from the volva of D.rubrovalvata were optimized by response surface methodology(Box-Behnken design)based on single factor experiments.The effervescent tablets were prepared with the extracted polysaccharides as raw materials by the method of acid-base separate granulation and mixed tableting.The results showed that the optimum extraction conditions were determined as follows:extraction time of 51 min,extraction temperature of 60℃,solid-to-liquid ratio of 1∶48(g/mL),and extraction power of 400 W.Under these conditions,the maximum polysaccharide yield of 13.86%was obtained.The optimum proportions of raw and auxiliary materials for the preparation of effervescent tablets were as follows:2%polysaccharides of D.rubrovalvata,45%effervescent disintegrants,acid-base(citric acid∶sodium bicarbonate)ratio of 1.5∶1,2%adhesives(3%PVP solution),2%lubricants(PEG 6000),and 1%sweetener(aspartame).The quality of the effervescent tablets was stable and the reproducibility was good,which conformed to the requirements of the 2020 edition of Chinese Pharmacopoeia.

关 键 词：红托竹荪 菌托 多糖 提取工艺 泡腾片 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]