Mechanisms of phototherapy of Alzheimer’s disease during sleep and wakefulness:the role of the meningeal lymphatics  

作　　者：Semyachkina‑Glushkovskaya Oxana Shirokov Alexander Blokhina Inna Fedosov Ivan Terskov Andrey Dubrovsky Alexander Tsoy Maria Elovenko Daria Adushkina Viktoria Evsukova Arina Telnova Valeria Tzven Anna Krupnova Valeria Manzhaeva Maria Dmitrenko Alexander Penzel Thomas Kurths Jürgen 

机构地区：[1]Institute of Physics,Humboldt University,Berlin 12489,Germany [2]Department of Biology,Saratov State University,Saratov 410012,Russia [3]Institute of Biochemistry and Physiology of Plants and Microorganisms,Russian Academy of Sciences,Saratov 410049,Russia [4]Charité–Universitätsmedizin Berlin,Berlin 10117,Germany [5]Department of Complexity Scienc,Potsdam Institute for Climate Impact Research,Potsdam 14473,Germany

出　　处：《Frontiers of Optoelectronics》2023年第3期57-76,共20页光电子前沿（英文版）

基　　金：We thank research center“Symbiosis”and immunochemistry laboratory IBPPM RAS for their support with immunofuorescence analysis and confocal microscopy within Project No.GR 121031100266-3;SGO,FI,SA,BI,TA,DA,ZM,ED,AV,EA,VV,TA,KV,MM,and MA were supported by grant(No.23-75-30001);the Russian Science Foundation,DA and ED were supported by Grant(No.21-75-10088);the Russian Science Foundation and by Grant from the Russian Ministry of Science and High Education(No.075-15-2022-1094).

摘　　要：With the increase in the aging population,the global number of people with Alzheimer’s disease(AD)progressively increased worldwide.The situation is aggravated by the fact that there is no the efective pharmacological therapy of AD.Photobiomodulation(PBM)is non-pharmacological approach that has shown very promising results in the therapy of AD in pilot clinical and animal studies.However,the mechanisms of therapeutic efects of PBM for AD are poorly understood.In this study on mice,we demonstrate that photodynamic efects of 5-aminolevulenic acid and laser 635 nm cause reduction of network of the meningeal lymphatic vessels(MLVs)leading to suppression of lymphatic removal of beta-amyloid(Aβ)from the right lateral ventricle and the hippocampus.Using the original protocol of PBM under electroencephalographic monitoring of wakefulness and sleep stages in non-anesthetized mice,we discover that the 7-day course of PBM during deep sleep vs.wakefulness provides better restoration of clearance of Aβfrom the ventricular system of the brain and the hippocampus.Our results shed light on the mechanism of PBM and show the stimulating efects of PBM on the brain lymphatic drainage that promotes transport of Aβvia the lymphatic pathway.The efects of PBM on the brain lymphatics in sleeping brain open a new niche in the study of restorative functions of sleep as well as it is an important informative platform for the development of innovative smart sleep technologies for the therapy of AD.

关 键 词：Alzheimer’s disease Photobiomodulation Brain lymphatics SLEEP MECHANISMS 

分 类 号：R749.16[医药卫生—神经病学与精神病学]