海藻糖对香菇多糖代谢及关键酶基因表达的影响  

Effects of trehalose on lentinan metabolism and expression of key enzyme genes of Lentinula edodes

作　　者：曹雨影 李艳 张莹 向泉桔 张凌子 辜运富 CAO Yuying;LI Yan;ZHANG Ying;XIANG Quanju;ZHANG Lingzi;GU Yunfu(College of Resources,Sichuan Agricultural University,Chengdu 611130,Sichuan,China)

机构地区：[1]四川农业大学资源学院,四川成都611130

出　　处：《菌物学报》2023年第11期2244-2256,共13页Mycosystema

基　　金：四川省科技计划项目(2021YFYZ0026)。

摘　　要：香菇多糖是从香菇中纯化提取出来,具有药用价值的一种高分子葡聚糖。本研究以香菇新808为研究对象,探究不同浓度外源海藻糖处理下的香菇菌丝生长速率、生物量、多糖含量、菌丝胞内外酶的活性及多糖合成关键酶基因表达量的差异。本研究发现在2.5mg/mL浓度海藻糖处理下,香菇菌丝生物量和多糖含量相较于对照组显著提高,菌丝生长速率与对照组间没有显著差异;参与调控菌丝多糖合成的胞外酶(木质素过氧化物酶、漆酶、纤维素酶、锰过氧化物酶和半纤维素酶)及胞内酶(葡萄糖激酶、磷酸葡萄糖变位酶、磷酸葡萄糖异构酶)的活性与对照相比均显著升高;多糖合成关键酶基因LENED_003770、LENED_004424、LENED_005589、LENED_010922和LENED_012941的表达量相对于对照组均显著上调,而LENED_009143基因表达量则明显下调。表明适宜浓度的外源海藻糖能有效提高香菇菌丝多糖合成量,有利于香菇多糖合成积累。Lentinan is a kind of polymer dextran purified and extracted from Lentinula edodes and has medicinal value.In this study,Lentinula edodes xin808 was used as the research object to explore the differences in the growth rate,biomass,polysaccharide content,mycelial and extracellular enzyme activity and gene expression of key enzymes for polysaccharide synthesis under different concentrations of exogenous trehalose treatment.The results showed that under the treatment of trehalose at a concentration of 2.5 mg/mL,the hyphal biomass and polysaccharide content in Lentinula edodes xin808 were significantly higher than those in the CK(control),while no significant difference was observed in the mycelium growth rate(P<0.05).The activities of extracellular enzymes such as lignin peroxidase,laccase,cellulase,manganese peroxidase and hemicellulase and intracellular enzymes including glucokinase,phosphoglucomutase and phospho-glucose isomerase involved in regulating the synthesis of mycelial polysaccharides of the strain xin808 were significantly higher than those of the control.The expression of LENED_003770,LENED_004424,LENED_005589,LENED_010922 and LENED_012941 genes of key enzymes for polysaccharide synthesis were significantly upregulated as compared with that of the control,while the expression of LENED_009143 gene was significantly down-regulated.The results proved that the exogenous trehalose with proper concentration could effectively increase the production of lentinan,being beneficial to the synthesis and accumulation of lentinan.

关 键 词：香菇 海藻糖 香菇多糖 合成关键酶 基因表达量 

分 类 号：S646.12[农业科学—蔬菜学]