木瓜蛋白酶制备北极甜虾虾壳多肽工艺研究  

Study on Preparation of Polypeptide from Shell of Arctic Sweet Shrimp with Papain

作　　者：刘雪凌[1] 林贝[1] 周莉莉 于翠[1] 亢娜 LIU Xue-ling;LIN Bei;ZHOU Li-li;YU Cui;KANG Na(Yanching Institute of Technology,Hebei Sanhe 065201,China)

机构地区：[1]燕京理工学院,河北三河065201

出　　处：《广州化工》2023年第14期88-90,共3页GuangZhou Chemical Industry

基　　金：廊坊市科技支撑计划项目《北极甜虾废弃物多肽钼螯合物的制备及活性研究》(No:2021013170)。

摘　　要：以北极甜虾虾壳为对象,研究从北极甜虾虾壳中提取多肽的工艺。通过单一实验因素实验,得到:酶浓度为10%、酶解时间为40 min、酶解温度为50℃、pH=4时多肽产率和水解度均最高。通过四因素三水平的正交试验得出最优工艺条件,即酶解时间为40 min、酶浓度为12.5%、酶解pH为4、酶解温度为50℃。该工艺可为有壳类海产废物制备活性肽积累更多的经验,为其实现工业化、实现废弃物有效利用提供有力支持。The technology of extracting polypeptide from the shell of Arctic sweet shrimp was studied.Through a single experimental factor experiment,it was finally obtained that the peptide yield and degree of hydrolysis were the highest when the enzyme concentration was 10%,the enzymatic hydrolysis time was 40 min,the enzymatic hydrolysis temperature was 50℃,and pH=4.Through the orthogonal test of four factors and three levels,the optimal technological conditions were obtained.Result showed the optimal technological conditions for the preparation of peptides were as follows:enzymatic hydrolysis time was 40 min,enzyme concentration was 12.5%,enzymatic hydrolysis pH was 4,and enzymatic hydrolysis temperature was 50℃.This process can accumulate more experience for the preparation of active peptides from shelled marine waste,and provide strong support for its industrialization and effective utilization of waste.

关 键 词：北极甜虾虾壳 多肽 木瓜蛋白酶 新工艺 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]