基于广泛靶向代谢组学技术的不同花色秋石斛中花青素差异分析  

Metabolomics Analysis of Anthocyanin in Different Flower Colors of Phalaenopsis-Type Dendrobium

作　　者：武美卿 廖易[2,3] 陆顺教 殷涵泰[1,2] 余文刚 李崇晖 WU Meiqing;LIAO Yi;LU Shunjiao;YIN Hantai;YU Wengang;LI Chonghui(College of Tropical Agriculture and Forestry,Hainan University,Haikou,Hainan 570228,China;Tropical Crop Germplasm Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture and Rural Affairs/Key Laboratory of Tropical Crops Germplasm Re-sources Genetic Improvement and Innovation of Hainan Province,Haikou,Hainan 571101,China;Hainan Provincial Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization,Danzhou,Hainan 571737,China)

机构地区：[1]海南大学热带农林学院,海南海口570228 [2]中国热带农业科学院热带作物品种资源研究所/农业农村部华南作物基因资源与种质创制重点实验室/海南省热带作物资源遗传改良与创新重点实验室,海南海口571101 [3]海南省热带观赏植物种质创新利用工程技术研究中心,海南儋州571737

出　　处：《热带作物学报》2023年第11期2167-2178,共12页Chinese Journal of Tropical Crops

基　　金：中央级公益性科研院所基本科研业务费专项(No.1630032022002);海南省自然科学基金高层次人才项目(No.321RC 648)。

摘　　要：为了深入研究不同花色秋石斛品种花青素合成途径的差异性,本研究利用广泛靶向代谢组学技术对蓝紫色、浅桃红色、红色、紫红色和深紫红色5个不同花色秋石斛品种的花青素苷组成和含量进行了分析。结果表明:从供试秋石斛中共鉴定出38个代谢产物,主要为矢车菊素(Cy)、芍药花素(Pn)、天竺葵素(Pg)、飞燕草素(Dp)和矮牵牛素(Pt)的糖苷及其酰基化衍生物。5个秋石斛品种中花青素苷组成和含量存在显著差异。代谢物差异分析表明,紫红色花中Cy和Dp型糖苷显著高于浅桃红色花(P<0.05);蓝紫色花的差异花青素中3种Dp型糖苷含量均显著高于其他颜色;仅筛选出1种Cy型糖苷在浅桃红色花中富集。绝大多数样品的Cy型糖苷含量最高,随着Cy和Dp及其衍生物含量增加,花色会加深并转向红紫色,推测Cy型糖苷使秋石斛偏向紫红色,而Dp糖苷赋予秋石斛蓝紫色调。基于以上结果推测秋石斛存在上述5种花青素苷的酰基化修饰途径,研究结果可为秋石斛花色形成机制和花色改良提供依据。In order to further investigate the anthocyanin biosynthetic pathway in Phalaenopsis-type Dendrobium(Den-Phals)varieties with different flower color,this study used widely targeted metabolomics technology to analyze the anthocyanin composition and content of five Den-Phals varieties with blue-purple,light peach-red,red,purple-red and deep purple-red floral color,respectively.The results showed that a total of 38 metabolites were identified from the tested Den-Phals,most of which were glycosides and acylated derivatives of cyanidin(Cy),peonidin(Pn),pelargonidin(Pg),delphinidin(Dp) and petunidin(Pt).There were significant differences in the composition and content of anthocyanin in five Den-Phals varieties.Metabolite difference analysis showed that Cy and Dp glycosides in purple flowers were significantly higher than those in light peach red flowers.The contents of three Dp-type glycosides in blue-purple flowers were significantly higher than those in other colored varieties.Only one Cy-type glycoside was screened out,and the content of which in light pink flowers was high.The content of Cy-type glycosides was the highest in most samples.With the increase of cyanidin and delphinidin and the derivatives,the color of flowers deepened and turned to red-purple.It was speculated that Cy-type glycosides made Den-Phals tend to purple while Dp glycosides gave Den-Phals a blue-purple tone.Based on the above results,it is speculated that there are acylation synthesis pathways of the above five anthocyanins in Den-Phals,which can provide a basis for flower color formation mechanism and flower color improvement of Den-Phals.

关 键 词：秋石斛 花色 代谢组 花青素 

分 类 号：S682.31[农业科学—观赏园艺]