基于pH响应聚合物的比色法检测牛奶中的鼠伤寒沙门氏菌  

Colorimetric detection of Salmonella typhimurium in milk based on pH responsive polymer

作　　者：白秀玉 杜鹏飞 王玥 王维婷[2,3] 柳尧波 马艳丽[2] 战汪涛 刘刚 李腾飞 BAI Xiu-Yu;DU Peng-Fei;WANG Yue;WANG Wei-Ting;LIU Yao-Bo;MA Yan-Li;ZHAN Wang-Tao;LIU Gang;LI Teng-Fei(School of Life Science and Food Engineering,Hebei University of Engineering,Handan 056038,China;Institute of Food and Nutrition Science and Technology,Shandong Academy of Agricultural Sciences,Shandong Provincial Key Laboratory of Agro-Products Processing Technology,Key Laboratory of Novel Food Resources Processing,Ministry of Agriculture and Rural Affairs,Jinan 250100,China;Yellow River Delta Modern Agriculture Research Center,Dongying 257000,China;Shandong Provincial Animal Husbandry Station,Jinan 250100,China)

机构地区：[1]河北工程大学生命科学与食品工程学院,邯郸056038 [2]山东省农业科学院农产品加工与营养研究所,山东省农产品精深加工技术重点实验室,农业农村部新食品资源加工重点实验室,济南250100 [3]东营黄河三角洲现代农业研究中心,东营257000 [4]山东省畜牧总站,济南250100

出　　处：《食品安全质量检测学报》2023年第21期116-123,共8页Journal of Food Safety and Quality

基　　金：河北省自然科学基金项目(C2019402379);黄三角国家农高区省级科技创新发展专项资金项目(2022SZX45);山东省农业科学院农业科技创新工程项目(CXGC2023B06);河北省重点研发计划项目(2232551D)。

摘　　要：目的基于pH响应聚合物构建一种用于检测牛奶中鼠伤寒沙门氏菌的比色传感器。方法通过溶剂诱导法,使用酚酞、适配体、牛血清白蛋白制备pH响应聚合物,基于适配体对鼠伤寒沙门氏菌的特异性结合与pH响应聚合物遇碱释放酚酞的比色反应建立鼠伤寒沙门氏菌比色检测方法,优化反应条件,并将其用于检测牛奶。结果该传感器在鼠伤寒沙门氏菌菌液浓度10^(2)~10^(7)CFU/mL范围内与吸光度呈现良好的线性关系,线性系数为0.982,检出限可达52 CFU/mL。将此法用于牛奶中鼠伤寒沙门氏菌的检测,加标回收率为83.2%~102.0%。结论该方法操作简便、结果可视化,为牛奶中鼠伤寒沙门氏菌的快速检测提供了一种新思路。Objective To construct a colorimetric sensor based on pH responsive polymer for the detection of Salmonella typhimurium in milk.Methods The solvent induction method was used to prepare pH responsive polymers using phenolphthalein,aptamer,and bovine serum protein.A colorimetric detection method for Salmonella typhimurium was established based on the specific binding of aptamers to Salmonella typhimurium and the colorimetric reaction of pH responsive polymers releasing phenolphthalein when exposed to alkali.The reaction conditions were optimized and applied to the detection of milk samples.Results The sensor exhibited a good linear relationship between the concentration and absorbance of Salmonella typhimurium in the concentration range of 10^(2)‒10^(7) CFU/mL,the linear correlation coefficient was 0.982 and the limit of detection was 52 CFU/mL.This method was used for the detection of Salmonella typhimurium in milk,and the recoveries were 83.2%‒102.0%.Conclusion This method is easy to operate and results are visualized,providing a new approach for the rapid detection of Salmonella typhimurium in milk.

关 键 词：适配体 pH响应聚合物 鼠伤寒沙门氏菌 比色法 

分 类 号：O657.3[理学—分析化学] TS252.7[理学—化学]