棉酚降解酵母的筛选及其在棉粕发酵中的应用  

Screening of gossypol degrading yeast and its application in cottonseed meal fermentation

作　　者：张国玉 柴娟 叶冬明 李新民 陈天明 蔡国林 Zhang Guoyu;Chai Juan;Ye Dongming;Li Xinming;Chen Tianming;Cai Guolin(Xinjiang Guannong Co.,Ltd.,Bayangol 841000;Xinjiang Guannong Testing Technology Co.,Ltd.,Bayangol 841000;Xinjiang Tianmu Biotechnology Co.,Ltd.,Hejing 841300;National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing,Jiangnan University,Wuxi 214122)

机构地区：[1]新疆冠农股份有限公司,巴州841000 [2]新疆冠农检测科技有限公司,巴州841000 [3]新疆天牧生物科技有限公司,和静841300 [4]江南大学粮食发酵与食品生物制造国家工程研究中心,无锡214122

出　　处：《粮食与食品工业》2023年第6期26-30,33,共6页Cereal & Food Industry

基　　金：“南疆反刍动物饲料发酵菌剂开发及示范”第二师铁门关市高新技术重点领域科技攻关项目(项目编号:2023GG2504)。

摘　　要：棉粕中存在的游离棉酚是限制其作为优质蛋白饲料应用的主要因素。采用含有0.3%游离棉酚的液体培养基来富集并分离获得酵母,通过考察酵母在以棉酚为唯一碳源培养基上的生长情况以及在棉粕固态发酵中棉酚的降解情况,获得了一株高效降解棉酚的产朊假丝酵母TM-2。棉粕经产朊假丝酵母TM-2发酵后,游离棉酚含量从578 mg/kg降低至128 mg/kg,粗蛋白含量从46.5%提高到53.3%,酸溶蛋白含量从4.4%提高至12.4%,抗营养因子棉籽糖含量从8.9%降低至0.8%,表明产朊假丝酵母TM-2显著改善棉粕的饲用品质。The presence of free gossypol in cottonseed meal is the main limitation of its application in protein feed.Yeast was enriched and isolated by liquid medium containing 0.3%free gossypol.The growth of yeast on the medium with gossypol as the sole carbon source and the degradation of gossypol in solid state fermentation of cottonseed meal were investigated,and then a strain TM-2 was found to be effective in gossypol degradation and identifeid as Candida utilis.Upon application of C.utilis TM-2 in cottonseed meal fermentation,the content of free gossypol decreased from 578 mg/kg to 128 mg/kg.The content of crude protein increased from 46.5%to 53.3%,and the content of acid-soluble protein increased from 4.4%to 12.4%.Protein quality was significantly improved,and the content of raffinose was decreased from 8.9%to 0.8%.These results demonstrate that C.utilis TM-2 can effectively improve the feed quality of cottonseed meal.

关 键 词：棉粕 棉酚 产朊假丝酵母 固态发酵 

分 类 号：TS201[轻工技术与工程—食品科学]