栽培地域和木屑对瓦尼桑黄子实体化学成分及生物活性的影响  

Effects of Cultivation Area and Sawdust Type on Chemical Composition and Biological Activities of Sanghuangporus vaninii Fruiting Bodies

作　　者：王雨阳 李正鹏[2] 陈万超[2] 张忠[2] 刘朋 吴迪[2] 李文[2] 杨焱[2] WANG Yuyang;LI Zhengpeng;CHEN Wanchao;ZHANG Zhong;LIU Peng;WU Di;LI Wen;YANG Yan(College of Food Science&Technology,Shanghai Ocean University,Shanghai 201306,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungal Processing,Shanghai 201403,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发中心,上海201403

出　　处：《食用菌学报》2023年第6期76-85,共10页Acta Edulis Fungi

基　　金：上海市科委农业领域项目(22N51900100);上海市农业科学院卓越团队建设计划[沪农科卓(2022)003]。

摘　　要：测定浙江海宁桑树,陕西榆林枣树、桑树以及湖北宜昌桑树、栎树木屑的化学成分,采用这五种木屑栽培瓦尼桑黄(Sanghuangporus vaninii),测定子实体多糖、总三萜、总酚、黄酮含量,并研究子实体醇提物抗氧化、体外抑制肿瘤细胞活性。结果表明:陕西榆林枣树木屑栽培的子实体多糖含量最高,为3.06%;浙江海宁桑树木屑栽培的子实体总三萜、总酚和黄酮含量均最高,分别为0.63%、16.09%、15.59%,且子实体醇提物对DPPH和ABTS自由基的清除率、对肿瘤细胞HepG-2、MCF-7、HeLa的抑制率最高。研究结果为利用栽培基质提高瓦尼桑黄子实体活性成分含量提供参考。Five kinds of sawdust,Haining mulberry sawdust(HN),Yulin jujube sawdust(SZ),Yulin mulberry sawdust(SS),Yuan’an mulberry sawdust(YS),and Yuan’an oak sawdust(YL),were analyzed for chemical composition and used to cultivate Sanghuangporus vaninii.The resultant fruiting bodies were analyzed for contents of polysaccharides,triterpenoids,flavonoids,total phenols,and monosaccharide composition.Alcohol extracts of the five kinds of S.vaninii fruiting bodies were determined for their antioxidant activity(ABTS and DPPH)and inhibitory activity on tumor cells in vitro.The results showed that fruiting bodies cultivated with SZ had the highest polysaccharide content(3.06%),and those cultivated with HN had the highest contents of total triterpenoids(0.63%),total phenols(16.09%)and total flavonoids(15.59%).The alcohol extract of fruiting bodies cultivated by HN showed the highest scavenging rate on DPPH and ABTS.It also showed the highest inhibition rate on HepG-2,MCF-7 and HeLa cells.These results provided a reference for enhancing bioactive components in S.vaninii through optimizing cultivation substrate.

关 键 词：瓦尼桑黄 木屑 活性成分 抗氧化活性 肿瘤细胞抑制率 

分 类 号：S567.3[农业科学—中草药栽培]