基于超高效液相色谱-三重四极杆质谱技术的冬瓜瓤化学成分分析  

Chemical composition analysis of wax gourd pith based on ultra-performance liquid chromatography-triple quadrupole mass spectrometry technology

作　　者：陆胜勇 傅曼琴[1] 徐玉娟[1] 谢大森[3] 余元善[1] 温靖[1] 肖更生[1,2] LU Shengyong;FU Manqin;XU Yujuan;XIE Dasen;YU Yuanshan;WEN Jing;XIAO Gengsheng(Sericultural&Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods,Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing,Guangzhou 510610,China;Zhongkai College of Agricultural Engineering of Light Industry Food Institute,Guangzhou 510225,China;Vegetable Research Institute,Guangdong Academy of Agricultural Sciences/Key Laboratory fog New Technology Research of Vegetables,Guangzhou 510640,China)

机构地区：[1]广东省农业科学院蚕业与农产品加工研究所/农业农村部功能食品重点实验室/广东省农产品加工重点实验室,广东广州510610 [2]仲恺农业工程学院轻工食品学院,广东广州510225 [3]广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广东广州510640

出　　处：《食品与发酵工业》2023年第23期307-314,共8页Food and Fermentation Industries

基　　金：广东省农业科学院食品营养与健康研究中心建设运行经费(XTXM202205);广东省农业科学院“十四五”农业优势产业学科团队(202109TD);广东省农业科学院人才项目(R2020PY-JX011);广东省现代农业产业技术体系创新团队建设项目(2022KJ110)。

摘　　要：采用超高效液相色谱-串联质谱的广泛代谢组学技术对冬瓜瓤的代谢物进行分离和鉴定,分析和描述冬瓜瓤的代谢物组成和丰度的信息。通过测定冬瓜皮、肉、瓤和籽4个部位的总黄酮、总酚及抗氧化能力发现,冬瓜瓤的总黄酮含量仅次于冬瓜肉,总酚含量则最高,抗氧化能力最强。因此,该研究选取冬瓜瓤作为研究对象,分析其化学成分,结果显示,冬瓜瓤中共分离鉴定出代谢物825个,初生代谢物451个(59.01%),次生代谢物374个(40.99%),其中氨基酸及其衍生物(114个,21.41%)、脂质(123个,13.28%)、有机酸(74个,10.82%)、糖及醇类(58个,6.68%)、核苷酸及其衍生物(62个,5.62%)、维生素(20个,1.22%)、酚酸类(161个,21.10%)、生物碱(57个,7.84%)、黄酮(92个,7.36%)、木脂素和香豆素(51个,3.63%)、萜类(13个,1.06%)。冬瓜瓤各类代谢物种类丰富,富集程度高,还具有显著的营养功能、活性作用及药效,其中相对含量≥0.5%的代谢物对冬瓜副产物营养和活性有重要贡献,该研究结果为冬瓜瓤副产物精深加工和利用提供理论依据。The metabolites from wax gourd pith were separated and identified by ultra-high performance liquid chromatography-tandem mass spectrometry-based extensive metabolomics technology and the information about the composition and abundance of metabolites in wax gourd pith was analyzed and described.By measuring the total flavonoids,total phenols,and antioxidant capacity of four parts of wax gourd skin,pulp,pith,and seed,it was found that the total flavone content of wax gourd pith was second only to that of wax gourd pulp,while the wax gourd pith had the highest total phenol content and the strongest antioxidant capacity.Therefore,the wax gourd pith was selected as the research object to analyze its chemical composition.Results showed that a total of 825 metabolites were isolated and identified from the pith of wax gourd,including 451(59.01%)primary metabolites and 374(40.99%)secondary metabolites,including amino acids and their derivatives(114,21.41%),lipids(123,13.28%),organic acids(74,10.82%),sugars and alcohols(58,6.68%),nucleotides and their derivatives(62,5.62%),vitamins(20,1.22%),phenolic acids(161,21.10%),alkaloids(57,7.84%),flavonoids(92,7.36%),lignans and coumarins(51,3.63%),and terpenes(13,1.06%).The flesh of wax gourd was rich in various metabolites with high abundance and also had significant nutritional function,active effect and efficacy.The metabolites with relative content≥0.5%had an important contribution to the nutrition and activity of wax gourd by-products.The results of this study provide a theoretical basis for the deep processing and utilization of wax gourd pulp by-products and improve the added value and economic benefits of the wax gourd industry.

关 键 词：冬瓜瓤 广泛靶向代谢组学 化学成分 营养物质 功能活性 

分 类 号：O657.63[理学—分析化学] TS255.2[理学—化学]