酿酒酵母启动子工程研究进展  

Research Progress of the Promoter Engineering in Saccharomyces cerevisiae

作　　者：蒋慧慧[1,2] 王强[2] 饶志明[2] 张显[2] JIANG Hui-hui;WANG Qiang;RAO Zhi-ming;ZHANG Xian(School of Biological and Environmental Engineering,Chaohu University,Hefei 238024,China;The Key Laboratory of Industrial Biotechnology of Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]巢湖学院生物与环境工程学院,合肥238024 [2]江南大学工业生物技术教育部重点实验室,无锡214122

出　　处：《中国生物工程杂志》2023年第11期78-91,共14页China Biotechnology

基　　金：国家重点研发计划(2020YFA0908300)资助项目。

摘　　要：启动子是起始基因转录的重要元件,启动子的功能与基因表达水平密切相关。启动子工程旨在研究启动子功能改造和定向进化,可以拓展和深化酿酒酵母启动子在合成生物学中的应用。基于酿酒酵母启动子的结构特征,重点综述了酿酒酵母启动子工程的策略和应用,即调控序列的敲除、传统启动子的随机突变、饱和突变、启动子杂交、合成最小化启动子骨架、转录因子结合位点的修饰和改造等技术原理和研究实例,并结合CRISPR/dCas9和人工智能工具在酿酒酵母启动子工程领域的最新进展,展望启动子工程在合成生物学领域的未来发展方向。Promoter is the most important element of initiation gene transcription and its function is closely related to the gene expression level.The promoter engineering aims to study the functional modification and directed evolution of promoters,which can expand and deepen the application of Saccharomyces cerevisiae promoters in synthetic biology.Based on the structural characteristics of S.cerevisiae promoters,this review focuses on the strategies and applications of S.cerevisiae promoter engineering,including regulatory sequence knockout,random mutation of traditional promoters,saturated mutation,promoter hybridization,synthesis of minimum promoter skeleton,and modification of transcription factor binding sites.In addition,the latest progress of CRISPR/dCas9 and artificial intelligence tools in the field of S.cerevisiae promoter engineering is introduced.The future development prospects of promoter engineering in the field of synthetic biology are also discussed.

关 键 词：酿酒酵母 转录调控 启动子改造 CRISPR/dCas9 人工智能 

分 类 号：Q815[生物学—生物工程]