寄主和产地对肉苁蓉活性成分及抗氧化能力的影响研究  

Effect of Different Hosts and Producing Areas on the Active Ingredients and Antioxidant Capacity of Cistanches deserticola

作　　者：安青 郭晔红[1] 安芳芳 马桃桃 贾存勤 AN Qing;GUO Ye-hong;AN Fang-fang;MA Tao-tao;JIA Cun-qin(Agronomy College,Gansu Agricultural University,Key Laboratory of Technological Innovation of Standard-ized Production of Traditional Chinese Medicines in Gansu Province,Gansu Provincial Key Lab of Arid Land Crop Science,Lanzhou Gansu 730070,China;Gansu Huiqin Biological Technology Co.,Ltd.,Baiyin Gan-su 730600,China)

机构地区：[1]甘肃农业大学农学院,甘肃省中药材规范化生产技术创新重点实验室,甘肃省干旱生境作物学重点实验室,甘肃兰州730070 [2]甘肃汇勤生物科技有限公司,甘肃白银730600

出　　处：《时珍国医国药》2023年第9期2236-2239,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(31860349);甘肃省科技计划项目(18YFlNA072,21CX6NH279)。

摘　　要：目的探究寄主和产地对肉苁蓉6种苯乙醇苷和粗多糖含量的影响,并评价抗氧化活性。方法采用HPLC法、苯酚-硫酸法测定肉苁蓉6种苯乙醇苷(松果菊苷、肉苁蓉苷A、管花苷A、毛蕊花糖苷、异毛蕊花糖苷、2’-乙酰基毛蕊花糖苷)和粗多糖含量,并测定其体外抗氧化活性(DPPH、ABTS清除能力和总抗氧化能力)。结果景泰、和田、阿克苏四翅滨藜-肉苁蓉6种苯乙醇苷总量分别较景泰梭梭-肉苁蓉提高了186.69%、214.64%、815.52%;随着样品浓度的增加,肉苁蓉醇提物浓度为2 mg/mL时,对DPPH、ABTS清除率均达到了90%以上;粗多糖浓度为10 mg/mL时,对DPPH、ABTS清除率分别达到了80%和99%以上;当样品浓度为2 mg/mL时,景泰梭梭-肉苁蓉、景泰、和田、阿克苏四翅滨藜肉苁蓉与VC总抗氧化能力分别为1.56、3.63、3.52、4.38、4.56μmol/mL。结论寄主和产地对肉苁蓉苯乙醇苷成分、粗多糖含量与抗氧化活性有显著性影响(P<0.05),且肉苁蓉抗氧化能力与样品浓度呈量效关系,四翅滨藜-肉苁蓉各方面表现均优于梭梭-肉苁蓉。这一研究结果为肉苁蓉作为天然的抗氧化剂和新寄主四翅滨藜-肉苁蓉的推广提供了参考价值。Objective To investigate the effect of different hosts and producing areas on the content of six phenylethanoid glyco-sides and crude polysaccharides in Cistanches deserticola and to evaluate the antioxidant activity.Methods HPLC and phenol-sulfuric acid method were used to determine the contents of six phenylethanoid glycosides(Echinacoside,Cistanoside A,Tubulo-side A,Verbascoside,Isoacteoside,2'-Acetylacteoside)and crude polysaccharides in C.deserticola,then the antioxidant activ-ities in vitro(DPPH、ABTS scavenging capacity and total antioxidant capacity)were detected.Results The total amount of six phe-nylethanoid glycosides in Atriplex canescens-C.deserticola from Jingtai,Hotan and Aksu increased by 186.69%,214.64%and 815.52%,respectively,compared with Haloxylon ammodendron-C.deserticola from Jingtai;With increasing sample con-centration,the alcoholic extract of C.deserticola achieved more than 90%clearance of DPPH and ABTS at a concentration of 2mg/mL;The crude polysaccharides at a concentration of 10 mg/ml achieved over 80%and 99%clearance of DPPH and ABTS,respectively;When the sample concentration is 2mg/mL,the total antioxidant capacities of H.amodendron-C.deserticola in Jingtai,A.canescens-C.deserticola in Jingtai,Hotan and Aksu with VC were 1.56,3.63,3.52,4.38 and 4.56μmol/mL,respectively.Conclusion Host and origin had significant effects(P<0.05)on phenylethanoid glycosides composition,crude polysaccharides content and antioxidant activity of C.deserticola,the antioxidant capacity of C.deserticola was correlated with the concentration of the sample,A.canescens-C.deserticola was superior to H.ammodendron-C.deserticola in all respects.The results of this study provide a reference value for the promotion of C.deserticola as a natural antioxidant and A.canescens as a new host for C.deserticola.

关 键 词：肉苁蓉 苯乙醇苷 粗多糖 抗氧化活性 总抗氧化能力 

分 类 号：R284.1[医药卫生—中药学]