紫苏叶多糖的双酶法提取工艺及其抗氧化活性研究  

A Study on Double Enzyme Extraction Process and Antioxidant Activity of Perilla Leaf Polysaccharide

作　　者：郎伍营 张佳怡 赵永平 LANG Wu-ying;ZHANG Jia-yi;ZHAO Yong-ping(College of Biopharmaceutical and Food Engineering,Shangluo University,Shangluo726000,Shaanxi;Electronic Information and Electrical College of Engineering,Shangluo University,Shangluo726000,Shaanxi)

机构地区：[1]商洛学院生物医药与食品工程学院,陕西商洛726000 [2]商洛学院电子信息与电气工程学院,陕西商洛726000

出　　处：《商洛学院学报》2023年第6期63-68,共6页Journal of Shangluo University

基　　金：陕西省大学生创新创业训练计划项目(S202211396071);商洛学院科研基金项目(20SKY020);陕西省重点研发计划项目(2021ZDLSF04-05)。

摘　　要：以紫苏叶为原料,采用纤维素酶和果胶酶优化紫苏叶多糖提取的工艺,并研究其抗氧化活性。紫苏叶经过粉碎,采用单因素试验,选择纤维素酶添加量、果胶酶添加量、酶解时间和酶解温度四个因素,利用正交试验法优化紫苏叶多糖的提取工艺条件。结果表明,正交试验确定的紫苏叶多糖提取的最佳工艺参数为纤维素酶添加量2.5%,果胶酶添加量2.5%,酶解时间60 min,酶解温度50℃,紫苏叶多糖提取率最大,可达到3.01%。紫苏叶多糖对DPPH自由基和羟基自由基均具有较好的清除能力。以期为紫苏叶产品深加工提供理论依据。Taking Perilla leaf as raw material,cellulase and pectinase were used to optimize the extraction process of polysaccharides from Perilla leaf,and their antioxidant activities were studied.After crushing Perilla leaves,the content of cellulase,the content of pectinase,the time of enzymatic hydrolysis and the temperature of enzymatic hydrolysis were selected as the main factors on the basis of single factor test,and the orthogonal experimental was used to determine the maximum extraction rate of polysaccharide from Perilla leaves.The results showed that the optimal extraction parameters were cellulase content 2.5%,pectinase content 2.5%,enzymolysis time 60 min,enzymolysis temperature 50℃,and the maximum extraction yield of polysaccharides from Perilla leaves could reach 3.01%.Perilla polysaccharide has good scavenging ability on DPPH free radical and hydroxyl free radical.To provide theoretical basis for deep processing of Perilla leaf products.

关 键 词：紫苏叶多糖 双酶法 正交试验 抗氧化活性 

分 类 号：S188[农业科学—农业基础科学]