臭氧微纳米气泡处理对黄豆芽“绿化”的影响及机制  

Effect of Ozone Micro-Nano-Bubbles Treatment on“Green”and the Mechanism in Soybean Sprout

作　　者：高红豆 史君彦[1] 刘海涛 左进华[1] 袁树枝 岳晓珍 王清[1] GAO Hongdou;SHI Junyan;LIU Haitao;ZUO Jinhua;YUAN Shuzhi;YUE Xiaozhen;WANG Qing(Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of the Vegetable Postharvest Treatment of Ministry of Agriculture,Beijing Key Laboratory of Fruits and Vegetable Storage and Processing,Beijing 100097,China;Beijing Fangyuan Ping’an Biotechnology Co.,Ltd.,Beijing 100097,China)

机构地区：[1]北京市农林科学院农产品加工与食品营养研究所,农业农村部蔬菜产后处理重点实验室,果蔬农产品保鲜与加工北京市重点实验室,北京100097 [2]北京方圆平安生物科技股份有限公司,北京100097

出　　处：《食品工业科技》2024年第1期295-302,共8页Science and Technology of Food Industry

基　　金：财政部和农业农村部:国家现代农业产业技术体系资助(CARS-23);北京市农林科学院协同创新中心建设(KJCX201915);北京市农林科学院创新能力建设专项(KJCX20220418,20210402,20200427和20210437);国家自然科学基金项目(31772022和32001763);北京市农林科学院青年基金(QNJJ202235)。

摘　　要：为研究臭氧微纳米气泡(ozone micro-nano-bubbles,Ozone MNBs)处理黄豆芽对LED白光照射下黄豆芽“绿化”的影响及其调控机制,本实验以黄豆芽为材料,采用4 mg/L Ozone MNBs处理黄豆芽,测定LED白光照射下黄豆芽生理品质,叶绿素合成和分解相关酶及物质变化。结果表明:与蒸馏水处理对照组相比,4 mg/L Ozone MNBs处理可以显著降低LED白光照射下黄豆芽的“绿化”现象,诱导提高黄豆芽中叶绿素降解酶[叶绿素酶(Chlase)、叶绿素降解过氧化物酶(Chl-POX)、脱镁螯合酶(MD)和脱镁叶绿素酶(PPH)]活性,降低了叶绿素合成前体物[δ-氨基乙酰丙酸(ALA)和尿卟啉原Ⅲ(UrogenⅢ)],叶绿素、叶绿素a(Chl a)和叶绿素b(Chl b)含量。降低了ADP、ATP和辅酶Ⅱ[NADP+和NADPH]含量。4 mg/L Ozone MNBs处理可影响黄豆芽叶绿素合成和分解相关酶的活性和相关物质的含量,对抑制LED白光照射下黄豆芽“绿化”有较好的效果。In order to explore the effect of ozone micro-nano-bubbles(Ozone MNBs)on“green”and the regulative mechanism in soybean sprout,this study took soybean sprout as the experimental material,treated with 4 mg/L Ozone MNBs and stored in white LED condition.Physical quality,synthesizing and decomposing of chlorophyll(enzyme activity and substance)were measured in soybean sprout.Compared with control group,4 mg/L Ozone MNBs treatment could significantly inhibit the“green”,enhance the activities of chlorophyllase(Chlase),chlorophyll degrading peroxidase(Chl-POX),Mg-dechelatase(MD)and pheophytinase(PPH).And it decreased the levels of precursors in chlorophyll synthesis[δ-aminolevulinic acid(ALA)and UrogenⅢ],chlorophyll,chlorophyll a and chlorophyll b.Additionally,it declined the content of ADP,ATP,NADP+and NADPH in soybean sprout.Thus,4 mg/L Ozone MNBs treatment affected the substance and enzyme activity of synthesizing and decomposing of chlorophyll,effectively hindered“green”in soybean sprout under white LED.

关 键 词：黄豆芽 臭氧微纳米气泡 LED 白光照射 叶绿素合成 机制 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]