复方石斛总黄酮提取及活性研究  

Study on Extraction and Activity of Total Flavonoids from Compound Dendrobium Officinale

作　　者：马丹[1] 尚书游 陈欣雨 付佳欣 阮军 MA Dan;SHANG Shuyou;CHEN Xinyu;FU Jiaxin;RUAN Jun(Institute of Agricultural Biotechnology,Jingchu University of Technology,Jingmen 448000,China;School of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430048,China;Hubei Jurui Biological Technology Co.,LTD.,Jingmen 431900,China)

机构地区：[1]荆楚理工学院农业生物技术研究所,湖北荆门448000 [2]武汉轻工大学食品科学与工程学院,湖北武汉430048 [3]湖北聚瑞生物科技有限公司,湖北荆门431900

出　　处：《荆楚理工学院学报》2023年第6期12-21,共10页Journal of Jingchu University of Technology

基　　金：湖北省教育厅支持企业技术创新发展项目(2021BLB211);湖北省教育厅科学研究计划项目(B2018233);荆楚理工学院科学研究项目(HX20230023);湖北省科技厅科学研究项目(2022BEC013)。

摘　　要：目的:以复方石斛(铁皮石斛-党参-黄芪-黄精=2∶3∶3∶2)为原料进行总黄酮的提取。方法:通过单因素实验和响应面法对提取工艺进行优化,并测定提取物体外抗氧化活性和体内降血糖活性。结果:复方石斛总黄酮的最优提取工艺条件为乙醇浓度60%,超声时间50min,液料比为35,总黄酮提取率为2.59%,测定其对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-trinitrophenylhydrazine,DPPH)、2,2-联氮-双-3-乙基苯并噻唑啉-6-磺酸(2,2-Binazo-bis-3-ethyl Benzothiazole line-6-sulfonic acid,ABTS+)、羟基自由基的半数抑制率分别为7.189μg/ml、1.288μg/ml、1.468μg/ml。采用线虫实验测定其体内降糖活性,当提取物制备成120 mg/ml的溶液时,有较好的降糖活性。结论:研究制备的复方石斛提取液具有较好的抗氧化和降糖作用。Purpose:The total flavonoids were extracted from compound Dendrobium officinale(Dendrobium officinale-Codonopsis pilosula-Huangqi-Huangjing=2:3:3:2).Method:The extraction process was optimized through single factor experiments and response surface methodology,and the antioxidant activity in vitro and hypoglycemic activity in vivo of the extracts were measured.Results:The results showed that the optimal extraction process conditions for total flavonoids in compound Dendrobium officinale were ethanol concentration of 60%,ultrasound time of 50 minutes,liquid-solid ratio of 35,and total flavonoid extraction rate of 2.59%.The half inhibition rates of 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH),2,2-biazo-bis-3-ethylbenzothiazoline-6-sulfonic acid(ABTS+),and hydroxyl radicals were determined to be 7.189μg/ml,1.288μg/ml,and 1.468μg/ml,respectively.The nematode experiment was used to determine its hypoglycemic activity in vivo,and when the extract was prepared into a 120 mg/ml solution,it had good hypoglycemic activity.Conclusion:The compound dendrobium extract prepared in the study has good antioxidant and hypoglycemic effects.

关 键 词：复方石斛 总黄酮 响应面法 抗氧化活性 降糖活性 

分 类 号：R284.2[医药卫生—中药学]