Anti-tumor Effect of Paclitaxel Enhanced by Psoralen at the Cellular Level  

作　　者：Yinghong HUANG Linqian CHEN Yaping WU Xian PENG Xuemei FANG Chunye LU Jiangcun WEI 

机构地区：[1]Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530201,China [2]Guangxi Medical University,Nanning 530021,China [3]Guangxi University of Chinese Medicine,Nanning 530001,China

出　　处：《Medicinal Plant》2023年第6期27-30,共4页药用植物：英文版

基　　金：Supported by the Natural Science Foundation Project of Guangxi University of Chinese Medicine(2020MS058);Youth Fund Project of Guangxi Natural Science Foundation(2022JJB140402);Basic Research Ability Improvement Project of Young and Middle-aged Teachers in Guangxi Universities(2022KY0278);"Youth Project"Talent Cultivation Project of Guangxi International Zhuang Medicine Hospital(2022001);High-level Key Discipline of Traditional Chinese Medicine(Zhuang Pharmacy)Construction Project of National Administration of Traditional Chinese Medicine(GZYYRJH[2022]226);Multidisciplinary Cross Innovation Team Project of Guangxi Traditional Chinese Medicine(GZKJ2309);"High-level Talent Cultivation and Innovation Team"Project of Guangxi University of Chinese Medicine(2022A008).

摘　　要：[Objectives]To explore the effect of psoralen combined with paclitaxel on the apoptosis of MCF-7 cells.[Methods]The effects of different concentrations of psoralen,paclitaxel,or the combination of psoralen and paclitaxel on cell viability were detected using CCK-8 assay kit.Cell cycle distribution and apoptosis after 24 h of psoralen(0.16,0.32,0.64 mmol/L),paclitaxel(0.1μmol/L),combined action of psoralen(0.32 mmol/L)and paclitaxel(0.1μmol/L)were detected using flow cytometry.[Results]Lower concentration of psoralen(0.04-0.32 mmol/L)showed no significant inhibitory effect on cells.After combined with paclitaxel,the inhibitory effect on MCF-7 cell proliferation was significantly higher than that of the group treated alone.Compared with the paclitaxel group,the cell apoptosis rate in the drug combination group was significantly increased.Different low concentrations of psoralen can block the cell cycle of MCF-7 at G 0/G 1 phase,while paclitaxel can block the cell cycle at G 2/M phase.After combined action,the number of cells blocked at G 2/M phase decreased.[Conclusions]Overall,the combined effect of psoralen and paclitaxel can enhance anti-tumor ability by inhibiting cell proliferation,inducing apoptosis,and blocking cell cycle.

关 键 词：PSORALEN PACLITAXEL Breast cancer APOPTOSIS 

分 类 号：R730[医药卫生—肿瘤]