Carbon Chain Length Determines Inhibitory Potency of Perfluoroalkyl Sulfonic Acids on Human Placental 3β-Hydroxysteroid Dehydrogenase 1:Screening,Structure-Activity Relationship,and In Silico Analysis  

作　　者：TANG Lu Ming MAO Bai Ping ZHANG Bing Ru LI Jing Jing TANG Yun Bing LI Hui Tao GE Ren Shan 

机构地区：[1]Department of Emergency Medicine,The Second Affiliated Hospital and Yuying Children Hospital of Wenzhou Medical University,Wenzhou 325000,Zhejiang,China [2]Department of Anesthesiology,The Second Affiliated Hospital and Yuying Children Hospital of Wenzhou Medical University,Wenzhou 325000,Zhejiang,China [3]Department of Obstetrics and Gynecology,The Second Affiliated Hospital and Yuying Children Hospital of Wenzhou Medical University,Wenzhou 325000,Zhejiang,China [4]Key Laboratory of Structural Malformations in Children of Zhejiang Province,Wenzhou 325000,Zhejiang,China

出　　处：《Biomedical and Environmental Sciences》2023年第11期1015-1027,共13页生物医学与环境科学（英文版）

摘　　要：Objective This study aimed to compare 9 perfluoroalkyl sulfonic acids(PFSA)with carbon chain lengths(C4–C12)to inhibit human placental 3β-hydroxysteroid dehydrogenase 1(3β-HSD1),aromatase,and rat 3β-HSD4 activities.Methods Human and rat placental 3β-HSDs activities were determined by converting pregnenolone to progesterone and progesterone secretion in JEG-3 cells was determined using HPLC/MS–MS,and human aromatase activity was determined by radioimmunoassay.Results PFSA inhibited human 3β-HSD1 structure-dependently in the order:perfluorooctanesulfonic acid(PFOS,half-maximum inhibitory concentration,IC50:9.03±4.83μmol/L)>perfluorodecanesulfonic acid(PFDS,42.52±8.99μmol/L)>perfluoroheptanesulfonic acid(PFHpS,112.6±29.39μmol/L)>perfluorobutanesulfonic acid(PFBS)=perfluoropentanesulfonic acid(PFPS)=perfluorohexanesulfonic acid(PFHxS)=perfluorododecanesulfonic acid(PFDoS)(ineffective at 100μmol/L).6:2FTS(1H,1H,2H,2H-perfluorooctanesulfonic acid)and 8:2FTS(1H,1H,2H,2H-perfluorodecanesulfonic acid)did not inhibit human 3β-HSD1.PFOS and PFHpS are mixed inhibitors,whereas PFDS is a competitive inhibitor.Moreover,1–10μmol/L PFOS and PFDS significantly reduced progesterone biosynthesis in JEG-3 cells.Docking analysis revealed that PFSA binds to the steroid-binding site of human 3β-HSD1 in a carbon chain length-dependent manner.All 100μmol/L PFSA solutions did not affect rat 3β-HSD4 and human placental aromatase activity.Conclusion Carbon chain length determines inhibitory potency of PFSA on human placental 3β-HSD1 in a V-shaped transition at PFOS(C8),with inhibitory potency of PFOS>PFDS>PFHpS>PFBS=PFPS=PFHxS=PFDoS=6:2FTS=8:2FTS.

关 键 词：3β-hydroxysteroid dehydrogenase 1 Docking analysis Perfluorooctanesulfonic acid PROGESTERONE STRUCTURE-ACTIVITYRELATIONSHIP 

分 类 号：R99[医药卫生—毒理学]