A near-infrared fluorescent probe for fast and precise imaging of senescent cells and ovarian cancer cells via trackingβ-galactosidase  

作　　者：Haiting Pan Xianzhi Chai Junji Zhang 

机构地区：[1]Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering,Feringa Nobel Prize Scientist Joint Research Center,School of Chemistry and Molecular Engineering,Frontiers Center for Materiobiology and Dynamic Chemistry,East China Universityof Science and Technology,Shanghai200237,China [2]School of Environmental and Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang 212100,China

出　　处：《Chinese Chemical Letters》2023年第12期456-459,共4页中国化学快报（英文版）

基　　金：supported by National Natural Science Foundation of China(Nos.22122803 and 21788102);the National Natural Science Foundation of Jiangsu Province(No.BK20220644).

摘　　要：Aging-related diseases are gradually becoming a major problem with the rapid development of aged population in human society.Although many fluorescent probes have been employed to diagnosis senescence via imaging senescence-associatedβ-galactosidase(SA-β-Gal),which is proved to be closely associated with senescent cells,the similar catalytic effectiveness of enzymatic reaction of ovarian cancer-associatedβ-Gal(OA-β-Gal)will interfere with imaging accuracy.Herein,a near-infrared(NIR)hemicyanine based fluorescent probe HCyXA-βGal was designed for light-up imaging of live cells containingβ-Gal.With the organelle-targeting morpholinyl and positive charge moieties,HCyxA-βGal was successfully applicated to image the difference of enzymatic location in senescent cells and ovarian cancer cells.Furthermore,inspired by the fast response performance,fast and precise imaging of the two cell lines was realized via covering another dimension of fluorescence signal:time-dependent intensity.

关 键 词：Β-GALACTOSIDASE Near-infrared fluorescent probe Senescent cell Precise imaging Fast response 

分 类 号：R737.31[医药卫生—肿瘤] O657.3[医药卫生—临床医学]