Chemical proteomic profling with photoaffinity labeling strategy identifies antimalarial targets of artemisinin  

作　　者：Peng Gao Jiayun Chen Peng Sun Jianyou Wang Huan Tang Fei Xia Liwei Gu Huimin Zhang Chen Wang Yin Kwan Wong Yinhua Zhu Chengchao Xu Jigang Wang 

机构地区：[1]Artemisinin Research Center,and Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China [2]Pharmaceutical College,Henan University,Kaifeng 475004,China [3]Shandong Academy of Chinese Medicine,Ji'nan 250014,China [4]Department of Nephrology,Shenzhen Key Laboratory of Kidney Diseases,Shenzhen Clinical Research Centre for Geriatrics,Shenzhen People's Hospital,The First Afiliated Hospital,Southern University of Science and Technology,Shenzhen 518020,China

出　　处：《Chinese Chemical Letters》2023年第12期460-465,共6页中国化学快报（英文版）

基　　金：supported by grants from the National Key Research and Development Program of China(Nos.2020YFA0908000 and 2022YFC2303600);the Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(No.ZYYCXTD-C-202002);the National Natural Science Foundation of China(Nos.82141001,82274182,82074098 and 82173914);the CACMS Innovation Fund(Nos.CI2021A05101 and CI2021A05104);:the Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(No.CI2021B014);the Science and Technology Foundation of Shenzhen(No.JCYj20210324115800001);the Science and Technology Foundation of Shenzhen(Shenzhen Clinical Medical Research Center for Geriatric Diseases);Establishment of Sino-Austria"Belt and Road"Joint Laboratory on Traditional Chinese Medicine for Severe Infectious Diseases and Joint Research(No.2020YFE0205100);the Fundamental Research Funds for the Central Public Welfare Research Institutes(Nos.ZZ14-YQ-050,ZZ14-YQ-051,ZZ14-YQ-052,ZZ14-FL-002,ZZ14-ND-010 and ZZ15-ND-10);Introduce innovative team projects of Jinan(No.202228029);Shenzhen Governmental Sustainable Development Fund(No.KCXFZ20201221173612034);Shenzhen Key Laboratory of Kidney Diseases(No.ZDSYS201504301616234);Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties(No.SZGSPO01).

摘　　要：Present research on the antimalarial mechanisms of artemisinin(ART)is mainly focused on covalent drug binding targets alkylated by free radicals,while non-covalent binding targets have rarely been reported.Here,we developed a novel photoaffinity probe of ART to globally capture and identify the antimalarial target proteins of ART through chemical proteomics.The results demonstrated that ART can bind to par-asite proteins by both covalent and non-covalent modification,and these may jointly contribute to the antimalarial effects.Our work enriches the research on the antimalarial targets of ART,and provides a new perspective for further exploring the antimalarial mechanism of ART.

关 键 词：ARTEMISININ ANTIMALARIAL Chemical proteomic Target identification Photoaffinity probe 

分 类 号：R531.3[医药卫生—内科学] O657.3[医药卫生—临床医学]