Extracellular ATP-activated hybridization chain reaction for accurate and sensitive detection of cancer cells  

作　　者：Lie Li Suping Li Jie Wang Xiaohong Wen Mei Yang Haiyan Chen Qiuping Guo Kemin Wang 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Biology,College of Chemistry and Chemical Engineering,Hunan University,State Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province,Changsha 410082,China

出　　处：《Chinese Chemical Letters》2023年第12期495-498,共4页中国化学快报（英文版）

基　　金：supported by the Natural Science Foundation of China(Nos.21877030,21735002 and 21778016).

摘　　要：Accurate and sensitive detection of caner cells is of significant importance for early diagnosis and treat-ment of cancer.Here,we developed an extracellular ATP-dctivated hybridization chain reaction(HCR)amplification strategy to meet this purpose.This strategy relies on three DNA probes,Apt-trigger,H1-AТP aptamer duplex and hairpin H2.The Apt-trigger probe consists of two com sequence for specific recognition of the target cells.and a trigger sequence for the HCR assembly.Theроnents:an aptamer duplex structure of H1-ATP aptamer causes the tochold in hairpin H1 to be hidden,preventing the strand-ent displacement reaction between haipin H1 and Apt-trigger.Upon activation with ATP the ATP aptamer will blnd to ATP to dissoci iate from hairpin H1,thus leading to an Apt-trigger-induced strand-displacement reaction and subsequent HCR with hairpin H2 on the target cell surface.Benefiting from aptamer recogni-tion and ATP-activated HCR amplification,this strategy can not only perform sensitive quantitative anal-ysis with a detection limit of 25 cells in 200 ul.of binding buffer,but also show desirable specificity and accuracy for identifylng target cells from control cells and mixed cell samples,Imporantly,this method retains stable and good perfor mance for target cell detection in 10%fetal bovine ser rum,den onstrating great potential for clinical diagnosis in complex biological matrices.Furthermore,this strategy can be adapted to detect various types of cancer cells by changing the corresponding aptamer sequence.

关 键 词：Cancer cells ATP APTAMER Hybridization chain reaction Fluorescence 

分 类 号：R730.43[医药卫生—肿瘤] O657.3[医药卫生—临床医学]