亚临界水法制备姜黄素纳米粒及其透膜性能分析  

Preparation and Membrane Permeability of Curcumin Nanoparticles by Subcritical Water Technology

作　　者：赵文英[1] 郑文迪 宋晓旭 王珧 刘蓓 陈蕾 朱庆书[1,2] ZHAO Wenying;ZHENG Wendi;SONG Xiaoxu;WANG Yao;LIU Bei;CHEN Lei;ZHU Qingshu(College of Chemical Engineering,Qingdao University of Science and Technology,Qingdao 266000,China;Gaomi Campus,Qingdao University of Science and Technology,Gaomi 261550,China)

机构地区：[1]青岛科技大学化工学院,山东青岛266000 [2]青岛科技大学高密校区,山东高密261550

出　　处：《食品科学》2023年第24期75-80,共6页Food Science

基　　金：山东省重点研发计划项目(2018GSF119008)。

摘　　要：采用亚临界水法制备姜黄素纳米粒,并评价其透膜性能。以纳米粒粒径分布、多分散指数以及Zeta电位为指标,考察影响制备姜黄素纳米粒的工艺条件,并对制得的纳米粒进行表征和体外透膜性能评价。结果表明:当亚临界水温度120℃、接收溶剂温度0℃、亚临界水/接收液体积比1∶3、以质量浓度0.04 g/100 mL乳糖为稳定剂、夹带剂乙醇体积分数30%时,制得姜黄素纳米粒粒径为166 nm,载药量为70.2%。傅里叶变换红外光谱显示姜黄素化学结构未发生改变;扫描电子显微镜显示姜黄素纳米粒呈均匀球形;差示扫描量热法分析表明纳米粒的结晶度下降。姜黄素纳米粒在体外具有良好的透膜性能,初始2 h内透膜速率为原料的25倍;在家兔体内也表现出良好的跨膜能力,能显著提升姜黄素的生物利用度。采用亚临界水法制备姜黄素纳米粒具有载药量高、透膜性能好、无需载体、工艺简单、绿色环保的优势。Curcumin(Cur)nanoparticles were prepared by subcritical water(SBCW)method and its membrane permeability was evaluated.The processing conditions for the preparation of curcumin nanoparticles were investigated in terms of its particle size distribution,polydispersity index(PDI)and zeta potential,and the prepared nanoparticles were characterized and evaluated for in vitro membrane permeability.The results showed that nanoparticles with a diameter of 166 nm and a drug loading of 70.2%were obtained under the conditions:SBCW temperature 120℃,receiving solvent temperature 0℃,subcritical water/receiving solution ratio 1:3(V/V),0.04 g/100 mL lactose as stabilizing agent,and 30%(V/V)ethanol as entrainer.Fourier transform infrared(FTIR)spectroscopy showed no structural changes in Cur.Scanning electron microscopy(SEM)showed that Cur nanoparticles were uniformly spherical in shape.Differential scanning calorimetry(DSC)analysis showed a decrease in the crystallinity of the nanoparticles.Cur nanoparticles exhibited excellent membrane permeability in vitro,and the permeation rate in the first two hours was 25 folds higher than that of free Cur.Furthermore,Cur nanoparticles exhibited good transmembrane capacity in rabbits and significantly enhanced the bioavailability of Cur.The preparation of Cur nanoparticles using subcritical water method has the advantages of high drug loading,good membrane permeability,no need for carriers,simple process,and green environmental protection.

关 键 词：姜黄素 亚临界水 制备 纳米粒 透膜性能 

分 类 号：TS202.3[轻工技术与工程—食品科学] R944.9[轻工技术与工程—食品科学与工程]