膨腹海马酶解物制备工艺优化与抗氧化活性  

Optimized Preparation and Antioxidant Activity of Enzymatic Hydrolysate from Hippocampus abdominals

作　　者：陈佩璐 李明 张嘉媛 邱峰岩 邱 罗友华[1,2] 戚欢阳 许光辉 CHEN Peiu;LI Ming;ZHANG Jiayuan;QIU Fengyan;QIU Xianzhu;LUO Youhua;QI Huanyang;XU Guanghui(Xiamen Key Laboratory of Natural Medicines Research and Development,Xiamen Institute of Pharmaceutical Research,Xiamen 361000,China;School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350000,China;Xiamen Xiaodeng Aquatic Technology Co.Ltd.,Xiamen 361000,China;Xiamen Enci Health Business Co.Ltd.,Xiamen 361000,China)

机构地区：[1]厦门市医药研究所厦门市天然药物研究与开发重点实验室,福建厦门361000 [2]福建中医药大学药学院,福建福州350000 [3]厦门小嶝水产科技有限公司,福建厦门361000 [4]厦门恩慈健康事业有限公司,福建厦门361000

出　　处：《肉类研究》2023年第10期22-29,共8页Meat Research

基　　金：厦门市扶持中医药发展专项资金资助项目(XWZY-2023-0605);厦门市海洋与渔业发展专项资金青年创新项目(23YYZP013QCA11);海洋生物资源开发重点项目(3502Z20226034);厦门市科技计划项目(3502Z20224004)。

摘　　要：以膨腹海马(Hippocampus abdominalis)为原料,1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除率为指标,优化海马酶解物提取工艺。根据DPPH自由基清除率,从碱性蛋白酶、风味蛋白酶、胰酶、木瓜蛋白酶、胰蛋白酶、胃蛋白酶中筛选出最优碱性蛋白酶进行工艺优化,在单因素试验基础上,通过Box-Behnken中心复合设计数学模型,采用响应曲面对酶解时间、pH值、酶底物比例(enzyme/substrate,E/S)进行考察,筛选出最佳的酶解工艺,并进行体外抗氧化能力的验证。结果表明,膨腹海马最佳酶解工艺为料液比1∶20、pH 11、E/S 7.581%、酶解温度50℃、酶解时间3 h,在此条件下DPPH自由基清除率能达64.62%。因此,响应曲面优化膨腹海马酶解物的提取工艺能够在较短时间内酶解得到抗氧化活性较高的活性肽。Objective:To optimize the preparation process of an enzymatic hydrolysate with 1,1-diphenyl-2-picrylhydrazyl(DPPH)scavenging activity from Hippocampus abdominalis.Methods:Alcalase,flavor protease,trypsin,papain,trypsin,and pepsin were screened for DPPH scavenging activity,and alcalase was found to be optimal.The optimization of the hydrolysis process was conducted using a combination of single factor experiments and response surface methodology(RSM)based on Box-Behnken design.Hydrolysis time,pH,and enzyme/substrate(E/S)ratio were considered as independent variables.The in vitro antioxidant capacity of the hydrolysate was verified.Results:The optimal hydrolysis conditions were determined as 1:20,11,7.581%,50℃and 3 h for solid/liquid ratio,pH,E/S ratio,temperature and time,respectively.The DPPH scavenging activity of the as-prepared hydrolysate was 64.62%.Conclusion:The optimized hydrolysis process can give peptides with high antioxidant activity from Hippocampus abdominalis.

关 键 词：膨腹海马 单因素试验 响应面试验 抗氧化活性肽 制备工艺 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]