壬基酚与辛基酚单独及联合暴露对大鼠肠道损伤的影响  

Separate and combined effects of nonylphenol and octylphenol exposure in rat intestinal injury

作　　者：黄丹菲[1] 杜鹏程 冷雪萍 王惠妹 李颖芝 高家铭 HUANG Danfei;DU Pengcheng;LENG Xueping;WANG Huimei;LI Yingzhi;GAO Jiaming(State Key Laboratory of Food Science and Resources,International Institute of Food Innovation Co.,Ltd.,Nanchang University,Nanchang 330047,China)

机构地区：[1]南昌大学食品科学与资源挖掘全国重点实验室,江西南大国创院食品科技有限公司,江西南昌330047

出　　处：《南昌大学学报（理科版）》2023年第6期570-577,共8页Journal of Nanchang University(Natural Science)

基　　金：江西省研究生创新专项资金项目(YC2019-S067);国家自然科学基金资助项目(31860471)。

摘　　要：两种典型环境内分泌干扰物——壬基酚(Nonylphenol,NP)与辛基酚(Octylphenol,OP),常常同时存在于自然环境中,经口摄入为它们进入人体内的主要途径。因此,NP与OP可能会对消化系统产生影响。目的:研究NP与OP单独及联合暴露对大鼠肠道损伤的影响。方法:将不同浓度的NP与OP单独或联合灌胃大鼠28天后,观察各组大鼠肠道氧化应激、通透性、炎症及肠道细胞凋亡情况。结果发现:NP与OP单独或联合暴露均会导致结肠组织结构破坏,紧密连接Claudin-1和Occludin蛋白表达水平下降,抗氧化酶活性发生改变,细胞因子IL-1β、TNF-α与IFN-γ表达水平上升,凋亡相关蛋白Caspase-3、Casspase-12、Bax和Bcl-2表达水平增加。结论:NP与OP单独或联合暴露均可通过改变肠道通透性、诱导大鼠肠道促炎细胞因子产生、降低抗氧化酶表达并诱导肠道细胞凋亡而对大鼠肠道造成不同程度的损伤。本研究对于全面评估环境内分泌干扰物联合暴露的健康风险,探索有效的预防干预措施,以及修订完善相关的卫生标准限值,具有重要意义。Nonylphenol(NP)and octylphenol(OP)are two typical environmental endocrine disruptors often coexisted in the environment,and their oral ingestion may impact the digestive system.OBJECTIVE:This study aimed to investigate the separate and combined effects of NP and OP exposure on rat intestinal injury.METHODS:Rats were administered varying concentrations of NP and OP individually or in combination via gavage for a duration of 28 days,and intestinal oxidative stress,inflammation,intestinal permeability and intestinal cell apoptosis in each group were observed.RESULTS:Individual or combined exposure to NP and OP led to structural damage to the colonic intestinal barrier,altered antioxidant enzyme activity in the colon,increased expression of cytokines IL-1β,TNF-αand IFN-γ,and increased expression of apoptotic proteins Caspase-3,Casspase-12,Bax and Bcl-2.CONCLUSIONS:The exposure to NP and OP,whether individually or in combination,resulted in increased pro-inflammatory cytokine production,decreased antioxidant enzyme expression,and induced apoptosis in the rat intestine,leading to different degrees of damage.This study may offer a theoretical foundation for the prevention and control of damage caused by environmental endocrine disruptors.

关 键 词：壬基酚 辛基酚 环境内分泌干扰物 肠道屏障 

分 类 号：R574[医药卫生—消化系统]