酸性蛋白酶AP-10分离纯化及其酶学性质  

Isolation,Purification,and Enzymatic Property of Acid Protease AP-10

作　　者：向珍蓉 余华顺[2] 龚大春 XIANG Zhenrong;YU Huashun;GONG Dachun(College of Biological&Pharmaceutical Sciences,China Three Gorges University,Yichang 443002,China;Angel Yeast Co.,Ltd.,Yichang 443002,China)

机构地区：[1]三峡大学生物与制药学院,湖北宜昌443002 [2]安琪酵母股份有限公司,湖北宜昌443002

出　　处：《化学与生物工程》2023年第12期27-30,43,共5页Chemistry & Bioengineering

基　　金：国家自然科学基金项目(21776162)。

摘　　要：采用活性炭吸附脱色、硫酸铵分级沉淀、脱盐和凝胶层析等技术对酸性蛋白酶AP-10浓缩液进行了分离纯化,并对其酶学性质进行了研究。结果表明,分离纯化后得到的酸性蛋白酶AP-10纯组分比酶活为5800 U·mg^(-1),纯化倍数为6倍;最适反应温度为40℃,热稳定范围为30~45℃;最适反应pH值为3.0,pH值稳定范围为3.5~6.5;Mn^(2+)对酸性蛋白酶AP-10具有强烈的激活作用,Fe 2+对酸性蛋白酶AP-10具有强烈的抑制作用。为拓展酸性蛋白酶AP-10的应用及开发高品质产品奠定了基础。In this paper,we purified the acid protease AP-10 concentrate by activated carbon decolorization,ammonium sulfate precipitation,desalination,and gel chromatography,and investigated its enzymatic property.The results show that the purified acid protease AP-10 with the specific enzyme activity of 5800 U·mg^(-1) and the purification fold of 6 is obtained.The optimal reaction temperature is 40℃,the thermal stability range is 30-45℃,the optimal reaction pH value is 3.0,and the stable range of pH value is 3.5-6.5.Mn^(2+)has a strong activating effect on the acid protease AP-10,while Fe 2+has a strong inhibitory effect on the acid protease AP-10.This study lays a foundation for expanding the application of acid protease AP-10 and developing high-quality products.

关 键 词：酸性蛋白酶AP-10 酶学性质 分离纯化 

分 类 号：Q556.9[生物学—生物化学] Q814