菌酶协同发酵甘草药渣制备蛋白饲料的工艺优化  

Fermentation optimization of protein feed preparation from licorice residue by bacteria and enzymes

作　　者：杨英歌[1] 吴江涛[1] 王娟[1] 王丹[1] 刘洁 YANG Ying-ge;WU Jiang-tao;WANG Juan;WANG Dan;LIU Jie

机构地区：[1]徐州生物工程职业技术学院,江苏徐州221006

出　　处：《饲料研究》2023年第24期81-85,共5页Feed Research

基　　金：徐州市科技项目(项目编号:KC22014)。

摘　　要：试验旨在开发新型蛋白饲料,采用单因素试验和响应面试验优化菌酶协同发酵甘草药渣制备蛋白饲料的工艺条件。结果显示,最佳发酵工艺条件为产朊假丝酵母用量7.36%、纤维素酶用量0.31%、木聚糖酶用量0.056%、料液比0.71 g/mL、初始pH值5.0、料层厚度7 cm、发酵温度37℃。在最佳发酵条件下,甘草药渣粗蛋白含量为25.65%,发酵后甘草药渣粗蛋白含量提高了13.36%;纤维素、半纤维素、木质素含量分别降低15.33%、2.32%、3.45%。研究表明,菌酶协同发酵甘草药渣提高了甘草药渣的营养价值,丰富了非常规蛋白饲料的资源。The aim of the experiment was to develop a new protein feed.A single factor test and a response surface test were used to optimize the process conditions of cooperating fermentation of licorice residue with bacterial enzymes to produce protein feed.The results showed that the optimum fermentation conditions were determined as follows:Candida utilis addition 7.36%,cellulase addition 0.31%,xylanase addition 0.056%,solid to liquid ratio 0.71 g/mL,initial pH 5.0,thickness of feed layer 7 cm,fermentation temperature 37℃.Under the optimal fermentation conditions,the crude protein content of licorice residue was 25.65%.After fermentation,the crude protein content increased by 13.36%.The content of cellulose,hemicellulose and lignin decreased by 15.33%,2.32%and 3.45%,respectively.The study indicates that the fermentation of licorice residue with bacterial enzymes improves the nutritional value of licorice residue and enriches the resources of unconventional protein feed.

关 键 词：菌酶协同 固体发酵 甘草药渣 蛋白饲料 

分 类 号：S816[农业科学—饲料科学]