Long non-coding RNA DPP10-AS1 represses the proliferation and invasiveness of glioblastoma by regulating miR-24-3p/CHD5 signaling pathway  

作　　者：JIWEI SUN LIANG XU YESEN ZHANG HAORAN LI JIE FENG XUEFENG LU JUN DONG 

机构地区：[1]Department of Neurosurgery,The Second Affiliated Hospital of Soochow University,Suzhou,215004,China [2]Department of Neurosurgery,The First Affiliated Hospital of Bengbu Medical College,Bengbu,233000,China

出　　处：《BIOCELL》2023年第12期2721-2733,共13页生物细胞（英文）

基　　金：supported through the Natural Science Foundation of Jiangsu Province(No.BK20201172);the Key Project of the Jiangsu Health Commission(No.ZDB2020016);the Jiangsu Province Key Research and Development Program:Social Development Project(No.BE2021653).

摘　　要：This investigation aimed to unveil new prospective diagnosis-related biomarkers together with treatment targets against glioblastoma.Methods:The expression levels of long non-coding RNA(lncRNA)DPP10-AS1 were assessed using real-time quantitative polymerase chain reaction(RT-qPCR)within both the patient tissue specimens and glioblastoma cell lines.The relationship between lncRNA DPP10-AS1 expression in glioblastoma and patient prognosis was investigated.Cell Counting Kit-8(CCK-8),transwell,and clonogenic experiments were utilized to assess tumor cells’proliferation,invasiveness,and migratory potentials after lncRNA DPP10-AS1 expression was up or down-regulated.Using an online bioinformatics prediction tool,the intracellular localization of lncRNA DPP10-AS1 and its target miRNA were predicted,and RNA-FISH verified results.A dual-luciferase reporter experiment validated the relationship across miR-24-3p together with lncRNA DPP10-AS1.MiR-24-3p expression within glioblastoma was identified through RT-qPCR,and potential link across miR-24-3p and lncRNA DPP10-AS1 was assessed using Pearson correlation analysis.Moreover,influence from lncRNA DPP10-AS1/miR-24-3p axis upon glioblastoma cell progression was assessed in vivo via a subcutaneous xenograft tumor model.Results:The expression of lncRNA DPP10-AS1 was notably reduced in both surgical specimens of glioblastoma and the equivalent cell lines.Low level of lncRNA DPP10-AS1 in glioblastoma is following poor prognosis.The downregulation of lncRNA DPP10-AS1 in glioblastoma cells resulted in enhanced cellular proliferation,migration,and invasion capabilities,accompanied by downregulated E-cadherin and upregulated vimentin and N-cadherin.Additionally,the observed upregulation of lncRNA DPP10-AS1 demonstrated a substantial inhibitory function upon proliferation,invasion,and migratory capabilities of LN229 cells.Subcellular localization disclosed that lncRNA DPP10-AS1 had a binding site that interacted with miR-24-3p.Upregulated miR-24-3p was detected in glioblastomas,displ

关 键 词：GLIOBLASTOMA lncRNA DPP10-AS1 miR-24-3p Chromodomain helicase DNA binding protein 5 

分 类 号：R730[医药卫生—肿瘤]