Low-molecular-weight fucoidan inhibits the proliferation of melanoma via Bcl-2 phosphorylation and PTEN/AKT pathway  

作　　者：MINJI PARK CHULHWAN BANG WON-SOO YUN YUN-MI JEONG 

机构地区：[1]T&R Biofab Co.,Ltd.,Seongnam-si,13487,Korea [2]Department of Dermatology,Seoul St.Mary’s Hospital,College of Medicine,The Catholic University of Korea,Seoul,02706,Korea [3]Department of Mechanical Engineering,Tech University of Korea,Si-heung City,15073,Korea

出　　处：《Oncology Research》2024年第2期273-282,共10页肿瘤学研究（英文）

基　　金：supported by the Priority Research Centers Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education,Science and Technology(Grant 2017R1A6A03015562 and RS-2023-00237386).

摘　　要：Fucoidan,a sulfate polysaccharide obtained from brown seaweed,has various bioactive properties,including anti-inflammatory,anti-cancer,anti-viral,anti-oxidant,anti-coagulant,anti-thrombotic,anti-angiogenic,and anti-Helicobacter pylori properties.However,the effects of low-molecular-weight fucoidan(LMW-F)on melanoma cell lines and three dimensional(3D)cell culture models are not well understood.This study aimed to investigate the effects of LMW-F on A375 human melanoma cells and cryopreserved biospecimens derived from patients with advanced melanoma.Ultrasonic wave was used to fragment fucoidan derived from Fucus vesiculosus into smaller LMW-F.MTT and live/dead assays showed that LMW-F inhibited cell proliferation in both A375 cells and patientderived melanoma explants in a 3D-printed collagen scaffold.The PTEN/AKT pathway was found to be involved in the anti-melanoma effects of fucoidan.Western blot analysis revealed that LMW-F reduced the phosphorylation of Bcl-2 at Thr 56,which was associated with the prevention of anti-apoptotic activity of cancer cells.Our findings suggested that LMW-F could enhance anti-melanoma chemotherapy and improve the outcomes of patients with melanoma resistance.

关 键 词：Low-molecular-weight fucoidan MELANOMA Patient-derived melanoma explants in a 3D-printed collagen scaffold Anti-melanoma effect PTEN-AKT-Bcl-2 network 

分 类 号：R735.9[医药卫生—肿瘤]