葡萄籽提取物化学成分分析及防腐效果评价  

Chemical composition analysis and preservative effect evaluation of grape seed extracts

作　　者：刘永衡 王葶 皇甫瑞娟 LIU Yongheng;WANG Ting;HUANGFU Ruijuan(School of Energy and Chemical Engineering,Ningxia Vocational Technical College of Industry and Commerce,Yinchuan 750021,China)

机构地区：[1]宁夏工商职业技术学院能源化工学院,宁夏银川750021

出　　处：《中国酿造》2023年第12期117-122,共6页China Brewing

基　　金：宁夏工商职业技术学院2021年院级科研重点项目(2021-02)。

摘　　要：为进一步明确葡萄籽化学成分及其提取物的防腐抑菌效果,该研究以宁夏贺兰山东麓产区酿酒葡萄籽为原料,采用非靶向超高压液相色谱串联二级质谱(UPLC-MS/MS)技术分别测定葡萄籽体积分数95%乙醇总浸提物(TE)及其正丁醇萃取物(TEN)的化学成分;此外,以山梨酸为对照,探究2种葡萄籽提取物对细菌(大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌)和真菌(尖孢镰刀菌、黑曲霉、柑橘青霉菌)的防腐抑菌效果。结果表明,两种提取物(TE及TEN)中共鉴定出60个化学成分,包括7种酚类、7种氨基酸、6种生物碱、6种有机酸、4种黄酮等成分。除枯草芽孢杆菌以外,质量浓度为1.5 g/L的TEN样品及TE样品对其他菌株的抑菌效果均为100%;此外,质量浓度为1 g/L的TEN样品对金黄色葡萄球菌及三种真菌抑菌效果为100%。因此,TEN防腐抑菌效果较好。To further definite the chemical composition of gra pe seeds and the antiseptic and antibacterial effects of their extracts,using the grape seeds from the East Helan Mountain Area in Ningxia as raw materials,the chemical components of grape seed volume fraction 95% ethanol extracts(TE) and its N-butanol extracts(TEN) were determined by non-targeted UPLC-MS/MS.In addition,using sorbic acid as positive control,the preservative effect of two extracts against three kinds of bacteria(Escherichia coli,Staphylococcus aureus,Bacillus subtilis) and three kinds of fungi(Fusarium oxysporum,Aspergillus niger,Penicillium italicum) were preliminarily evaluated.The results showed that a total of 60 chemical components were identified from two grape seed extracts(TE and TEN),including 7 phenols,7 amino acids,6 alkaloids,6 organic acids,4 flavonoids and other components.Except for B.subtilis,the antibacterial effect of TEN and TE samples with mass concentration of 1.5 g/L against other strains was 100%.In addition,the TEN samples with mass concentration of 1 g/L showed 100% antibacterial effect on S.aureus and three kinds of fungi.Therefore,TEN had better preservative effects.

关 键 词：葡萄籽提取物 化学成分 超高压液相色谱串联二级质谱 防腐效果 

分 类 号：TS261.1[轻工技术与工程—发酵工程]