Macrophage-secreted exosomes inhibit breast cancer cell migration via the miR-101-3p/DLG5 axis  

作　　者：Yu Liu Chao-Qun Wang Yong-Kang Zhu Jia-Fang Xu Si-Qi Yin Qing-Jie Hu Rui-Qi Yang 

机构地区：[1]First Clinical Medical College,Nanjing University of Chinese Medicine,Nanjing 210001,China [2]Department of Breast Surgery,The First Affiliated Hospital of Hainan Medical University,Haikou 570102,China [3]Department of Nuclear Medicine,Hainan General Hospital,Hainan Affiliated Hospital of Hainan Medical University,Haikou 570311,China [4]Reproductive Medicine Center,The First Affiliated Hospital of Hainan Medical University,Haikou 570102,China

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2023年第12期532-538,共7页亚太热带生物医学杂志（英文版）

基　　金：supported by the Key Research and Development Program of Hainan Province(ZDYF2020139,ZDYF2018158);the Science and Technology Funding Project of Hainan Province(821MS129).

摘　　要：Objective:To investigate the role of macrophages in regulating breast cancer cell migration and its related mechanisms.Methods:Human leukemia monocytic cell line THP-1-secreted exosomes were isolated using multi-step ultracentrifugation and verified using nanoparticle tracking analysis.Differentially expressed miRNAs were identified using RNA sequencing.Overexpression of inhibitors of hsa-miR-101-3p in breast cancer MDA-MB-231 cells was performed by infecting their lentiviral constructs.The luciferase reporter assay was used to evaluate the interaction of DLG5 and miR-101.DGL5 expression was detected using qRT-PCR and Western blot analyses.Results:The migration of breast cancer cells was significantly inhibited after addition of exosomes.RNA sequencing results showed that miR-101-3p expression was significantly upregulated.Targetscan analysis predicted that miR-101-3p could target DLG5,and this prediction was verified using the luciferase assay.The addition of the miR-101-3p precursor significantly increased the expression of miR-101-3p,and the mRNA and protein levels of DLG5 were suppressed.In contrast,inhibiting the expression of miR-101-3p increased the mRNA and protein levels of DLG5.Furthermore,the scratch assay showed that inhibiting miR-101-3p could promote the migration of MDA-MB-231 cells.Conclusions:Macrophage exosomes can inhibit the migration of breast cancer cells,and increasing the expression of miR-101-3p to inhibit DLG5 expression may play an important role in this process,which needs further investigation.

关 键 词：MICRO-RNA Tumor-associated macrophages EXOSOMES Breast cancer DLG5 

分 类 号：R737.9[医药卫生—肿瘤]