液相色谱-串联质谱法测定鱼肉中青霉素类药物残留量的不确定度评定  

Uncertainty Evaluation of Determination of Penicillin Residues in Fish by Liquid Chromatography-Tandem Mass Spectrometry

作　　者：李歆 汤小苇 张于 LI Xin;TANG Xiaowei;ZHANG Yu(Huai’an Product Quality Supervision and Inspection Center,Huai’an 223001,China)

机构地区：[1]淮安市产品质量监督综合检验中心,江苏淮安223001

出　　处：《食品安全导刊》2023年第36期52-59,共8页China Food Safety Magazine

摘　　要：目的:通过高效液相色谱法-串联质谱法的应用合理评定检测鱼肉中青霉素类药物残留量的不确定度。方法:参考《食品安全国家标准水产品中青霉素类药物多残留的测定液相色谱-串联质谱法》(GB 31656.12—2021)、《化学分析中不确定度的评估指南》(CNAS—GL 006:2019)中的规定与要求,分析青霉素类药物在测定过程中的各种不确定度来源。结果:当11种青霉素类药物残留量测定结果在21.07~109.86 mg·kg^(-1)时,其扩展不确定度4.64~20.26 mg·kg^(-1)(k=2)。结论:影响不确定度的关键性因素主要有标准溶液的配制过程、标准溶液线性拟合和定容样液的过程。Objective:To Evaluation of uncertainty in the determination of penicillin residues in fish by high-performance liquid chromatography-tandem mass spectrometry.Method:According to the provisions and requirements in GB 31656.12—2021,CNAS—GL006:2019,the sources of uncertainty in the determination of penicillins were analyzed.Result:When the results of 11 kinds of penicillin residues were 21.07~109.86 mg·kg^(-1)the expanded uncertainty is 4.64~20.26 mg·kg^(-1)(k=2).Conclusion:The main factors affecting the uncertainty are the preparation process of standard solution,linear fitting of standard solution and the process of constant volume sample solution.

关 键 词：液相色谱-串联质谱(LC-MS/MS) 鱼肉 青霉素类药物 不确定度 

分 类 号：O657.63[理学—分析化学] TS254.7[理学—化学]