Intermittent F-actin Perturbations by Magnetic Fields Inhibit Breast Cancer Metastasis  

作　　者：Xinmiao Ji Xiaofei Tian Shuang Feng Lei Zhang Junjun Wang Ruowen Guo Yiming Zhu Xin Yu Yongsen Zhang Haifeng Du Vitalii Zablotskii Xin Zhang 

机构地区：[1]High Magnetic Field Laboratory of CAS(CHMFL),CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology,HFIPS,Hefei,Anhui 230031,P.R China [2]Institutes of Physical Science and Information Technology,Anhui University,Hefei,Anhui,230601,P.R.China [3]Science Island Branch of Graduate School,University of Science and Technology of China,Hefei,Anhui 230031,P.R China [4]Institute of Physics of the Czech Academy of Sciences,Prague,Czech Republic [5]International Magnetobiology Frontier Research Center,Science Island,Hefei 230031,P.R.China.

出　　处：《Research》2023年第4期321-335,共15页研究（英文）

基　　金：This work was supported by the National Natural Science Foundation of China(U21A20148,52007185,and 31900506);International Partnership Program of Chinese Academy of Sciences(116134KYSB20210052);the CASHIPS Director’s Fund(2021YZGH04,BJPY2021A06,and YZJJZX202014);A portion of this work was supported by the High Magnetic Field Laboratory of Anhui Province(AHHMFX-2021-05);the Heye Health Technology Chong Ming Project under award number(HYCMP2021010);Operational Programme Research,Development and Education as well as financed by the European Structural and Investment Funds and the Czech Ministry of Education,Youth and Sports through Project No.SOLID21-CZ.02.1.01/0.0/0.0/16_019/0000760;the CAS president’s international fellowship initiative(PIFI)grant(2022VMA0009)。

摘　　要：F-actin(filamentous actin)has been shown to be sensitive to mechanical stimuli and play critical roles in cell attachment,migration,and cancer metastasis,but there are very limited ways to perturb F-actin dynamics with low cell toxicity.Magnetic field is a noninvasive and reversible physical tool that can easily penetrate cells and human bodies.Here,we show that 0.1/0.4-T 4.2-Hz moderate-intensity low-frequency rotating magnetic field-induced electric field could directly decrease F-actin formation in vitro and in vivo,which results in decreased breast cancer cell migration,invasion,and attachment.Moreover,lowfrequency rotating magnetic fields generated significantly different effects on F-actin in breast cancer vs.noncancerous cells,including F-actin number and their recovery after magnetic field retrieval.Using an intermittent treatment modality,low-frequency rotating magnetic fields could significantly reduce mouse breast cancer metastasis,prolong mouse survival by 31.5 to 46.0%(P<0.0001),and improve their overall physical condition.Therefore,our work demonstrates that low-frequency rotating magnetic fields not only can be used as a research tool to perturb F-actin but also can inhibit breast cancer metastasis through F-actin modulation while having minimum effects on normal cells,which reveals their potential to be developed as temporal-controlled,noninvasive,and high-penetration physical treatments for metastatic cancer.

关 键 词：METASTASIS INVASION cancer 

分 类 号：R730[医药卫生—肿瘤]