四株猪源乳杆菌的分离鉴定及生物学特性研究  

Isolation and identification and biological characteristics of four porcine lactic acid bacteria

作　　者：张誓育 李咏[1] 朱润 吴银宝[1] 米见对 ZHANG Shiyu;LI Yong;ZHU Run;WU Yinbao;MI Jiandui(South China Agricultural University,Guangzhou,Guangdong Province 510642,China)

机构地区：[1]华南农业大学,广东广州510642

出　　处：《中国饲料》2024年第1期44-50,共7页China Feed

基　　金：广东省自然科学基金(2019A1515011022)。

摘　　要：本试验旨在筛选优质猪源乳杆菌,为养猪生产过程中微生态制剂的开发奠定基础。通过16S rRNA分析鉴定,本试验从健康成年猪粪中成功分离出四株猪源乳杆菌,并对这四株菌的生长性能、生理生化特性、耐酸、耐高温、耐胆盐和耐药性进行了综合测试。结果表明:ZLgz1227具有最优的综合性能,其耐胆盐性能最强,过胃存活率分别高于其他三种菌株110.56%、44.3%、4.98%。研究表明这四株菌都有制成益生菌制剂的潜能,为实际生产提供一些后备益生菌菌株参考。The purpose of this experiment was to screen high quality Lactobacillus from pigs and lay a foundation for the development of microecological agents in pig production.Four strains of Lactobacillus isolated from pig manure were identified by 16Sr RNA analysis.The growth performance,physiological and biochemical characteristics,acid resistance,high temperature resistance,bile salt resistance and drug resistance of the four strains were tested The results showed that ZLgz1227 had the best comprehensive properties,and its bile salt resistance was the strongest,and its gastric survival rate was 110.56%44.3%4.98%higher than the other three strains.The study indicated that all the four strains had the potential to make probiotic preparations in order to provide some reference for the actual production of probiotic strains.

关 键 词：乳杆菌 抗逆性 益生特性 

分 类 号：S816.3[农业科学—饲料科学]