HS-GC-IMS结合ROAV分析烤烟烟叶“糯米香”特征成分  

Analysis of Characteristic“Glutinous Rice”-like Aroma-active Odorants in Flue-cured Tobacco Leaves Based on HS-GC-IMS Combined with ROAV

作　　者：李舒畅 李军[3] 高宪辉 刘文霖[5] 常诗瑜 欧泽洁 庞雪莉[1] 孔凡玉[1] LI Shuchang;LI Jun;GAO Xianhui;LIU Wenlin;CHANG Shiyu;OU Zejie;PANG Xueli;KONG Fanyu(Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081,China;China Tobacco Shandong Industry Co.,Ltd.,Jinan 250014,China;Yunnan Tobacco Leaf Company,Kunming 650218,China;Tobacco Science Institute of Guizhou Province,Guiyang 550003,China)

机构地区：[1]中国农业科学院烟草研究所,青岛266101 [2]中国农业科学院研究生院,北京100081 [3]山东中烟工业有限责任公司,济南250014 [4]云南省烟草烟叶公司,昆明650218 [5]贵州省烟草科学研究所,贵阳550003

出　　处：《中国烟草科学》2023年第6期75-83,共9页Chinese Tobacco Science

基　　金：中国农业科学院科技创新工程(ASTIP-TRIC07);中国烟草总公司云南省公司科技项目(2021530000241011);中国烟草总公司贵州省公司科技项目(2021520000240044)。

摘　　要：为准确鉴定烤烟中“糯米香”特征风味化合物,以云南典型“糯米香”(NM)和非“糯米香”(CK)的云烟87烟叶为材料,采用顶空-气相色谱-离子迁移谱(HS-GC-IMS)技术分析其挥发物的组成及相对含量;基于挥发物的相对气味活度值(ROAV)和偏最小二乘法判别分析(OPLS-DA)等统计学方法,鉴定了“糯米香”特征关键标志风味化合物。结果表明,从供试烟叶共鉴定出57种挥发物(包括单体和二聚体),其中2-乙酰基-1-吡咯啉(糯米香),苯乙醛(果香),壬醛(柑橘香)以及2,3-丁二酮(奶甜香)被鉴定为“糯米香”与非“糯米香”烤烟烟叶间的差异性特征风味化合物,其中2-乙酰基-1-吡咯啉是“糯米香”特征的关键贡献化合物,其余3种化合物对烤烟“糯米香”特征香韵具有重要的修饰作用。本研究可能为烤烟“糯米香”特征风味精准调控及评价方法开发提供数据基础。In order to accurately identify the potent odorants in flue-cured tobacco(FCT)leaves with“glutinous rice”-like characteristic,different Yunyan 87 tobacco leaves with or without typical“glutinous rice”-like characteristic from Yunnan province were used as materials in this study.Firstly,volatile compositions and their relative content was analyzed by headspace-gas chromatography-ion migration spectroscopy(HS-GC-IMS).Then,the differential volatile markers between typical“glutinous rice”-like and non-“glutinous rice”-like tobacco leaves samples and the key chemical contributors to the“glutinous rice”-like characteristic were identified based on the relative odor activity value(ROAV)combined with orthogonal partial least squares discriminant analysis(OPLS-DA).A total of 57 common odorants(including monomers and dimers)were identified.2-Acetyl-1-pyrroline(“glutinous rice”-like),benzene acetaldehyde(fruity),nonanal(citrus-like),and 2,3-butanedione(sweet,buttery)are characterized as differential flavor compounds between typical“glutinous rice”-like and non-“glutinous rice”-like tobacco leaves samples,among which 2-acetyl-1-pyrroline is a key contributor to“glutinous rice”-like aroma,the other three compounds play an important role in modifying“glutinous rice”-like characteristic flavor of FCT.The results herein would provide chemical basis for the accurate regulation and the evaluation method development for“glutinous rice”-like characteristic odorants in FCT.

关 键 词：烤烟 糯米香 挥发性关键风味化合物 顶空-气相色谱-离子迁移谱 相对气味活度值 2-乙酰基-1-吡咯啉 

分 类 号：TS411[农业科学—烟草工业]