Targeted screening of an anti-inflammatory polypeptide from Rhopilema esculentum Kishinouye cnidoblasts and elucidation of its mechanism in alleviating ulcerative colitis based on an analysis of the gut microbiota and metabolites  

作　　者：Ziyan Wang Qiuyue Shi Ying Feng Jiaojiao Han Chenyang Lu Jun Zhou Zhonghua Wang Xiurong Su 

机构地区：[1]State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Ningbo University,Ningbo 315000,China [2]School of Marine Science,Ningbo University,Ningbo 315000,China [3]Key Laboratory of Aquaculture Biotechnology(Ningbo University),Ministry of Education,Ningbo University,Ningbo 315000,China [4]College of Life Sciences,Tonghua Normal University,Tonghua 134000,China [5]Shandong Beiyou Biotechnology Co.,Ltd.,Weifang 261000,China

出　　处：《Food Science and Human Wellness》2024年第3期1336-1347,共12页食品科学与人类健康（英文）

基　　金：sponsored by the National Key R&D Program of China (2018YFD0901102);the Natural Science Foundation of Zhejiang Province (LQ22D060002);the Fund of State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products (ZS20190105);the Fundamental Research Funds for the Provincial Universities of Zhejiang (SJLY2021015);the K.C.Wong Magna Fund of Ningbo University。

摘　　要：Ulcerative colitis(UC)is a recurrent inflammatory bowel disease that imposes a severe burden on families and society.In recent years,exploiting the potential of marine bioactive peptides for the treatment of diseases has become a topic of intense research interest.This study revealed the mechanism underlying the protective effect of the dominant polypeptide PKKVV(Pro-Lys-Lys-Val-Val)of Rhopilema esculentum cnidoblasts against DSS-induced UC through a combined analysis of the metagenome and serum metabolome.Specifically,the polypeptide composition of R.esculentum cnidoblasts was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF/TOF-MS).Molecular docking showed that the dominant peptide PKKVV could bind better with tumor necrosis factor-α(TNF-α)than the original ligand.Subsequent animal experiments suggested that PKKVV could modulate disorganized gut microorganisms in mice with UC;affect serum metabolites through the arachidonic acid,glycerophospholipid and linoleic acid metabolism pathways;and further alleviate UC symptoms.This study provides a reference for the comprehensive development of marine bioactive substances and nonpharmaceutical treatments for UC.

关 键 词：Ulcerative colitis Rhopilema esculentum Kishinouye Cnidoblasts Marine bioactive polypeptides METAGENOME Serum metabolome 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]