Lutein-stevioside nanoparticle attenuates H_(2)O_(2)-induced oxidative damage in ARPE cells  

作　　者：Zhuqing Dai Meimei Nie Ye Chen Jiangfeng Song Yayuan Xu Zhongyuan Zhang Guodong Zhang Shumo Yan Xing Zhang Dajing Li 

机构地区：[1]Institute of Agro-Product Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China [2]Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base,Ministry of Science and Technology,Nanjing 210014,China [3]Jiangsu Aland Nutrition Co.,Ltd.,Taizhou 214500,China [4]Aland Nutrition Taizhou Co.,Ltd.,Taizhou 225300,China

出　　处：《Food Science and Human Wellness》2024年第3期1628-1635,共8页食品科学与人类健康（英文）

基　　金：the National Natural Science Foundation of China (31801541);the Independent Innovation Fund Project of Agricultural Science and Technology in Jiangsu Province (CX (22)3065);Major Scientific and Technological Achievements Transformation Project of Taizhou (SCG 202105);the Taizhou Science and Technology Support Plan (TN202106)。

摘　　要：In order to improve the bioavailability of lutein(LUT),a novel lutein-stevio side nanoparticle(LUT-STE)were prepared previously,but the information about LUT-STE on protecting of eye health was limited.This study investigated the effect of LUT-STE on antioxidant activity of H_(2)O_(2)-induced human retinal pigment epithelial(ARPE)cells.LUT and LUT-STE(final concentration of 5μg/mL)significantly enhanced cell viability from(74.84±5.10)%to(81.92±10.01)%(LUT)and(89.33±4.34)%(LUT-STE),and inhibited the cell apoptosis(P<0.05).After pretreatment with LUT-STE in ARPE cells,the levels of superoxide dismutase(SOD),catalase(CAT)and glutathion peroxidase(GSH-Px)in ARPE cells were significantly increased(P<0.05),the contents of reactive oxygen species(ROS)and malondialdehyde(MDA)were decreased.In addition,the vascular endothelial growth factor(VEGF)levels were inhibited by 13.61%and 17.39%,respectively,pretreatment with LUT and LUT-STE.Western blotting results showed that the pretreatment with LUT-STE inhibited the expression of caspase-9 and caspase-3 and up-regulated Bcl-2/Bax pathway to inhibit H_(2)O_(2)-induced apoptosis.In summary,the novel delivery LUT-STE had more pronounced inhibitory effect on H_(2)O_(2)-induced damage in human ARPE cells.

关 键 词：LUTEIN STEVIOSIDE Antioxidant Human retinal pigment epithelial cell Mechanism 

分 类 号：TS201.4[轻工技术与工程—食品科学]