Ascophyllum nodosum and Fucus vesiculosus ameliorate restenosis via improving inflammation and regulating the PTEN/PI3K/AKT signaling pathway  

作　　者：Crystal Ngofi Zumbi Chun-Hsu Pan Hui-Yu Huang Chieh-Hsi Wu 

机构地区：[1]Program in Clinical Drug Development from Herbal Medicine,College of Pharmacy,Taipei Medical University,Taipei 11031,China [2]Program in Drug Discovery and Development Industry,College of Pharmacy,Taipei Medical University,Taipei 11031,China [3]School of Pharmacy,Taipei Medical University,Taipei 11031,China [4]Graduate Institute of Metabolism and Obesity Sciences,Taipei Medical University,Taipei 11031,China

出　　处：《Food Science and Human Wellness》2024年第3期1711-1728,共18页食品科学与人类健康（英文）

基　　金：supported by the research grants from the Ministry of Science and Technology of Taiwan (MOST108-2320B-038-040-MY3 and MOST 111-2320-B-038-049);the National Science and Technology Council (NSTC111-2320-B-038-049)。

摘　　要：Restenosis is a common complication following coronary angioplasty.The traditional use of seaweeds for health benefits has increasingly been explored,however few studies exist reporting its protective effects on the development of restenosis and gut dysbiosis.The aim of this study was to investigate the potential of seaweed extracts(SE) of Ascophyllum nodosum and Fucus vesiculosus in inhibiting intimal hyperplasia in a rat model of restenosis and its underlying mechanisms in macrophages and vascular smooth muscle cells(vSMCs).16S rRNA sequencing was done to investigate the regulatory effect of SE on the gut microbiome of injured rats.As indicated by the results,SE significantly inhibited the progression of intimal hyperplasia in vivo,attenuated inflammation in macrophages and could inhibit the proliferation,dedifferentiation and migration of vSMCs.It was observed through immunoblotting assays that treatment with SE significantly upregulated PTEN expression in macrophages and inhibited the upregulation of PI3K and AKT expression in vSMCs.Meanwhile,according to the 16S rRNA gene sequencing analysis,supplementation with SE modulated gut microbiota composition in injured rats.In conclusion,SE could ameliorate intimal hyperplasia by inhibiting inflammation and vSMCs proliferation through the regulation of the PTEN/PI3K/AKT pathway and modulating the gut microbiome.

关 键 词：Ascophyllum nodosum Fucus vesiculosus PTEN/PI3K/AKT RESTENOSIS MACROPHAGE Vascular smooth muscle cells Gut microbiota 

分 类 号：TS201.4[轻工技术与工程—食品科学]