Mobile genetic elements facilitate the transmission of antibiotic resistance genes in multidrug-resistant Enterobacteriaceae from duck farms  

作　　者：Xin’er Zheng Dingting Xu Jinchang Yan Min Qian Peng Wang Davood Zaeim Jianzhong Han Daofeng Qu 

机构地区：[1]School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310035,China [2]The Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 311100,China [3]Agricultural and rural Bureau of Wangdian Town,Xiuzhou District,Jiaxing 314011,China

出　　处：《Food Science and Human Wellness》2024年第2期729-735,共7页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China(32172188);Science and Technology Cooperation Project of ZheJiang Province(2023SNJF058-3)。

摘　　要：Multidrug-resistant(MDR)Enterobacteriaceae critically threaten duck farming and public health.The phenotypes,genotypes,and associated mobile genetic elements(MGEs)of MDR Enterobacteriaceae isolated from 6 duck farms in Zhejiang Province,China,were investigated.A total of 215 isolates were identified as Escherichia coli(64.65%),Klebsiella pneumoniae(12.09%),Proteus mirabilis(10.23%),Salmonella(8.84%),and Enterobacter cloacae(4.19%).Meanwhile,all isolates were resistant to at least two antibiotics.Most isolates carried tet(A)(85.12%),blaTEM(78.60%)and sul1(67.44%)resistance genes.Gene co-occurrence analysis showed that the resistance genes were associated with IS26 and integrons.A conjugative IncFII plasmid pSDM004 containing all the above MGEs was detected in Proteus mirabilis isolate SDM004.This isolate was resistant to 18 antibiotics and carried the blaNDM-5 gene.MGEs,especially plasmids,are the primary antibiotic resistance gene transmission route in duck farms.These findings provide a theoretical basis for the rational use of antibiotics in farms which are substantial for evaluating public health and food safety.

关 键 词：Duck farm Mobile genetic element Antibiotic resistance gene PLASMID Food safety 

分 类 号：R155.5[医药卫生—营养与食品卫生学] TS201.6[医药卫生—公共卫生与预防医学]