Aptasensing biosynthesized phosphatidylserine with a AuNPs nanozyme-based colorimetric aptasensor  

作　　者：Sai Wang Rui Ma Chengqiang Li Ling Zhang Haiyang Zhang Xuehan Li Xiangzhao Mao 

机构地区：[1]Qingdao Key Laboratory of Food Biotechnology,College of Food Science and Engineering,Ocean University of China,Qingdao 266404,China [2]Key Laboratory of Biological Processing of Aquatic Products,China National Light Industry,Qingdao 266404,China [3]Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China

出　　处：《Food Science and Human Wellness》2024年第2期823-829,共7页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China(31922072);the Natural Science Foundation of Shandong Province(ZR2020JQ15);the Taishan Scholar Project of Shandong Province(tsqn201812020)。

摘　　要：Sensitive monitoring of the target products during the biosynthesis process is crucial,and facile analytical approaches are urgently needed.Herein,phosphatidylserine(PS)was chosen as the model target,a colorimetric aptasensor was developed for the rapid quantitation in biosynthesis samples.A chimeric aptamer was constructed with two homogeneous original PS aptamers.Specific recognition between the chimeric aptamer and PS results in the desorption of aptamer from the surface of the AuNPs nanozyme,and the peroxidase-like enzymatic activity of the AuNPs nanozyme was weakened in a relationship with the different concentrations.The developed aptasensor performed well when applied for analyzing PS in biosynthesis samples.The aptasensor offers good sensitivity and selectivity,under optimal conditions,achieving monitoring and quantitation of PS in the range of 2.5-80.0μmol/L,with a limit of detection at 536.2 nmol/L.Moreover,the aptasensor provides good accuracy,with comparison rates of 98.17%-106.40%,when compared with the HPLC-ELSD.This study provides a good reference for monitoring other biosynthesized products and promoting the development of aptamers and aptasensors in real-world applications.

关 键 词：PHOSPHATIDYLSERINE Chimeric aptamer AuNPs nanozyme Colorimetric aptasensor 

分 类 号：TS201.2[轻工技术与工程—食品科学] O657[轻工技术与工程—食品科学与工程]