A ratiometric fluorescent probe for hypoxanthine detection in aquatic products based on the enzyme mimics and fluorescence of cobalt-doped carbon nitride  

作　　者：Xin Wang Chengyi Hong Zhengzhong Lin Zhiyong Huang 

机构地区：[1]College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,China

出　　处：《Food Science and Human Wellness》2024年第2期879-884,共6页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China(21804050);the National Key R and D Program of China(2018YFD0901003);the Science and Technology Planning Project of Xiamen,China(3502Z20183031);the Fujian Provincial Fund Project(2018J01432);the Xiamen Science and Technology Planning Project,China(3502Z20183031)。

摘　　要：A ratiometric fluorescent probe for hypoxanthine(Hx)detection was established based on the mimic enzyme and fluorescence characteristics of cobalt-doped graphite-phase carbon nitride(Co doped g-C_(3)N_(4)).In addition to emitting strong fluorescence,the peroxidase activity of Co doped g-C_(3)N_(4)can catalyze the reaction of O-phenylenediamine and H_(2)O_(2)to produce diallyl phthalate which can emit yellow fluorescence at 570 nm.Through the decomposition of Hx by xanthine oxidase,Hx can be indirectly detected by the generating hydrogen peroxide based on the measurement of fluorescent ratio I(F_(570)/F_(370)).The linear range was 1.7-272.2 mg/kg(R^(2)=0.997),and the detection limit was 1.52 mg/kg(3σ/K,n=9).The established method was applied to Hx detection in bass,grass carp,and shrimp,and the data were verified by HPLC.The result shows that the established probe is sensitive,accurate,and reliable,and can be used for Hx detection in aquatic products.

关 键 词：Co doped g-C_(3)N_(4) Ratiometric fluorescent probe HYPOXANTHINE FRESHNESS Aquatic products 

分 类 号：O657.3[理学—分析化学] TS254.7[理学—化学]