Lactobacillus plantarum AR113 attenuates liver injury in D-galactose-induced aging mice via the inhibition of oxidative stress and endoplasmic reticulum stress  

作　　者：Yongjun Xia Yujie Gong Xiangna Lin Yijin Yang Xin Song Guangqiang Wang Zhiqiang Xiong Yangyang Qian Zhuan Liao Hui Zhang Lianzhong Ai 

机构地区：[1]School of Health Science and Engineering,Shanghai Engineering Research Center of Food Microbiology,University of Shanghai for Science and Technology,Shanghai 200093,China [2]College of Life Sciences,Linyi University,Linyi 276000,China [3]Department of Gastroenterol.,Digestive Endoscopy Center,Changhai Hospital,Shanghai 200082,China

出　　处：《Food Science and Human Wellness》2024年第2期885-897,共13页食品科学与人类健康（英文）

基　　金：supported by the National Science Fund for Distinguished Young Scholars(32025029);the Shanghai Education Committee Scientific Research Innovation Projects(2101070007800120);the Yili Health Science Foundation of Chinese Institute of Food Science and Technology(2021-Y06);the Shanghai Engineering Research Center of food microbiology program(19DZ2281100)。

摘　　要：Probiotics could effectively eliminate excess reactive oxygen species(ROS)generated during aging or lipid metabolism disorders,but their mechanism is unclear.The major purpose of this study was to investigate the mechanism of Lactiplantibacillus plantarun AR113 alleviating oxidative stress injury in the D-galactose induced aging mice.The result showed that pretreatment with L.plantarun AR113 significantly relieving H_(2)O_(2)induced cytotoxicity in HepG2 cells by maintain cell membrane integrity and increasing antioxidant enzyme activities.In D-galactose induced aging mice,L.plantarun AR113 could significantly attenuate liver damage and inflammatory infiltration by promoting endogenous glutathione(GSH)synthesis and activating the Nrf2/Keap1 signaling pathway in mice,and increasing the expression of regulated phaseⅡdetoxification enzymes and antioxidant enzymes.Further analysis shown that gavage of L.plantarun AR113 could significantly reduce the expression of G protein-coupled receptor 78(GPR78)and C/EBP homologous protein(CHOP)proteins,and promote the restoration of endoplasmic reticulum(ER)homeostasis,thereby activating cell anti-apoptotic pathways.These results were also confirmed in H_(2)O_(2)-treated HepG2 experiments.It indicated that L.plantarun AR113 could inhibit D-galactose-induced liver injury through dual inhibition of ER stress and oxidative stress.L.plantarun AR113 have good application potential in anti-aging and alleviating metabolic disorders.

关 键 词：Lactiplantibacillus plantarun AR113 HEPG2 D-GALACTOSE Oxidative stress ER stress 

分 类 号：TS201.4[轻工技术与工程—食品科学] TS201.3[轻工技术与工程—食品科学与工程]