Tb^(3+)-nucleic acid probe-based label-free and rapid detection of mercury pollution in food  

作　　者：Xuhan Xia Chenxi Zhou Yulin Zhu Yi Dong Qiang He Mohammad Rizwan Khan Yuanlong Chi Rosa Busquets Ruijie Deng Yao Ren 

机构地区：[1]College of Biomass Science and Engineering,Healthy Food Evaluation Research Center,Sichuan University,Chengdu 610065,China [2]Department of Chemistry,College of Science,King Saud University,Riyadh 11451,Saudi Arabia [3]School of Life Sciences,Pharmacy and Chemistry,Kingston University London,Kingston Upon Thames KT12EE,UK

出　　处：《Food Science and Human Wellness》2024年第2期993-998,共6页食品科学与人类健康（英文）

基　　金：financially supported by National Natural Science Foundation of China(22074100);the Young Elite Scientist Sponsorship Program by CAST(YESS20200036);the Researchers Supporting Project Number RSP-2021/138;King Saud University,Riyadh,Saudi Arabia;Technological Innovation R&D Project of Chengdu City(2019-YF05-31702266-SN);Sichuan University-Panzhihua City joint Project(2020CDPZH-5)。

摘　　要：Mercury is a threatening pollutant in food,herein,we developed a Tb^(3+)-nucleic acid probe-based label-free assay for mix-and-read,rapid detection of mercury pollution.The assay utilized the feature of light-up fluorescence of terbium ions(Tb^(3+))via binding with single-strand DNA.Mercury ion,Hg^(2+)induced thymine(T)-rich DNA strand to form a double-strand structure(T-Hg^(2+)-T),thus leading to fluorescence reduction.Based on the principle,Hg^(2+)can be quantified based on the fluorescence of Tb^(3+),the limit of detection was 0.0689μmol/L and the linear range was 0.1-6.0μmol/L.Due to the specificity of T-Hg^(2+)-T artificial base pair,the assay could distinguish Hg^(2+)from other metal ions.The recovery rate was ranged in 98.71%-101.34%for detecting mercury pollution in three food samples.The assay is low-cost,separation-free and mix-to-read,thus was a competitive tool for detection of mercury pollution to ensure food safety.

关 键 词：Mercury pollution Food safety Nucleic acid probe LABEL-FREE TERBIUM 

分 类 号：TS207.51[轻工技术与工程—食品科学]