Epicatechin attenuates lead(Pb)-induced cognitive impairment in mice:regulation on Nrf2 signaling pathway,and interference on the interaction between Pb with albumin  

作　　者：Dai Cheng Qianqian Yu Kexin Zhu Dingdong Bu Zijian Wu 

机构地区：[1]State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science&Technology,Tianjin 300457,China [2]School of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China

出　　处：《Food Science and Human Wellness》2024年第2期1065-1078,共14页食品科学与人类健康（英文）

基　　金：supported by the National Key Research and Development Program of China under Grant(2022YFF1102800);the Graduate Scientific Research Innovation Project of Tianji(2022SKY109);the Project of Tianjin Science and Technology Program(22JCYBJC00360);the Project of Tianjin Science and Technology Program(21ZYJDJC00060)。

摘　　要：Epicatechin(EC)was used in this study to antagonize the cognitive dysfunction caused by lead(Pb)exposure in mice.Eight-week-old male Kunming mice were treated with PbCl_(2)(20 mg/kg)and/or EC(50 mg/kg)by gavage administration for 4 weeks.Morris water maze test showed that EC could improve memory dysfunction induced by Pb.EC antagonized Ca^(2+)overload,activated Nrf2 signaling pathway and reduced the accumulation of Pb in the brain and serum,which suggested that EC might alter Pb distribution in mice.In vitro,spectroscopic analysis,potentiometric titration and docking studies were applied to inquiry into the interaction between bovine serum albumin(BSA)and Pb^(2+)in presence or absence of EC.EC was proved to chelate Pb^(2+)and reduced the interaction between BSA and Pb^(2+).In summary,EC might protect Pb-induced cognitive impairment by activating Nrf2 signaling pathway,and suppressing Pb accumulation via interference on the binding of Pb to albumin.

关 键 词：LEAD EPICATECHIN Bovine serum albumin Interaction Oxidative stress 

分 类 号：TS201.4[轻工技术与工程—食品科学]