基于右旋糖酐酶制备小麦多孔淀粉及其特性  

Wheat Porous Starch:Preparation and Characteristics with Dextranase-Catalyzed Rotation

作　　者：王博岩 李强 吴一卓 章均哲 吴旭东 张磊 吕明生 王淑军 WANG Boyan;LI Qiang;WU Yizhuo;ZHANG Junzhe;WU Xudong;ZHANG Lei;LÜMingsheng;WANG Shujun(Jiangsu Provincial Key Laboratory of Marine Biological Resources and Environment,Jiangsu Ocean University,Lianyungang 222005,China;Key Laboratory of Marine Biotechnology of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,China;Haiyang City Market Supervision Administration,Yantai 265100,China)

机构地区：[1]江苏省海洋生物资源与环境重点实验室,江苏海洋大学,江苏连云港222005 [2]江苏省海洋生物技术重点实验室,江苏海洋大学,江苏连云港222005 [3]海阳市市场监督管理局,山东烟台265100

出　　处：《食品工业科技》2024年第3期123-129,共7页Science and Technology of Food Industry

基　　金：国家自然科学基金(32172154);国家重点研发计划项目(深海新型生物酶制剂创制与产业化,2022YFC2805101)。

摘　　要：生物酶法制备多孔淀粉条件温和、工艺简单和绿色环保。本研究选用特异性水解α-1,6糖苷键的右旋糖酐酶制备小麦多孔淀粉。通过X-晶体射线衍射、傅里叶红外光谱、扫描电镜和粒度分析仪对淀粉的结构进行表征,同时测定了淀粉膨胀率、吸水和吸油率、药品负载率和体外溶出等指标。结果表明:在温度为50℃条件下处理16 h、小麦淀粉的表面形成孔洞,淀粉粒径减小,多孔淀粉的晶型结构无显著变化。多孔淀粉的比容积为2.11±0.02 cm^(3)/g、溶解度为9.02%±0.21%、膨胀度达到了9.91%±0.22%、吸水率和吸油率分别提高到80.12%和78.23%;多孔淀粉与姜黄素、甲磺酸达比加群酯、制霉菌素和氧氟沙星四种药品在160:1的比例下负载率平均达到了75.07%。体外试验显示,小麦多孔淀粉负载姜黄素的释放率显著降低。在胃液中2.5 h的释放率约为30%。在肠液中5 h的释放率约为40%。研究结果为小麦多孔淀粉的制备和应用提高的依据。The preparation of porous starch by biological enzyme method is mild conditions,simple process,and environmentally sustainable.This study employed a dextranase with specific hydrolysis ofα-1,6-glycosidic bonds ability to prepare porous wheat starch.The structure of the starch was analyzed by X-ray diffraction,Fourier-transform infrared spectroscopy,scanning electron microscopy,and particle size analysis.The rates of starch swelling,oil and water absorption,and drug loading,as well as solubility,were measured.The results showed the formation of pores on the starch surface after treatment at 50℃for 16 h,together with reduced particle sizes,while the crystal structure of the starch did not change significantly.The specific volume of the porous starch was 2.11±0.02 cm^(3)/g,and the solubility was 9.02%±0.21%.Swelling reached 9.91%±0.22%while the water and oil absorption rates were increased to 80.12%and 78.23%,respectively.The loading rates of curcumin,dabigatran mesylate,nystatin,and ofloxacin in a 160:1 ratio reached an average of 75.07%.In vitro experiments showed that the release rate of curcumin loaded onto wheat porous starch was significantly reduced.The release rate in stomach for 2.5 h was approximately 30%,and it was approximately 40%in intestine for 5 h.The research results provided a basis for the preparation and application of wheat porous starch.

关 键 词：小麦淀粉 右旋糖酐酶 多孔淀粉 药物负载 

分 类 号：TS231[轻工技术与工程—粮食、油脂及植物蛋白工程]