两株代谢咖啡酸人肠道来源菌株的筛选、鉴定及其代谢过程初探  

Screening and Identification of Two Human Intestinal Strains Metabolizing Caffeic Acid and Exploring Their Metabolic Processes

作　　者：杨雯 胡海明 刘洪涛 魏晓博 刘慧燕 方海田[1] YANG Wen;HU Haiming;LIU Hongtao;WEI Xiaobo;LIU Huiyan;FANG Haitian(School of Food Science and Engineering,Ningxia Key Laboratory of Food Microbiology Application Technology and Safety Control,Ningxia University,Yinchuan 750021,China;College of Basic Medical Sciences,Hubei University of Chinese Medicine,Wuhan 430065,China)

机构地区：[1]宁夏大学食品科学与工程学院,宁夏食品微生物应用技术与安全控制重点实验室,宁夏银川750021 [2]湖北中医药大学基础医学院,湖北武汉430065

出　　处：《食品工业科技》2024年第3期137-145,共9页Science and Technology of Food Industry

基　　金：宁夏重点研发计划项目(2018BBF02008);宁夏食品微生物应用技术与安全控制重点实验室平台建设项目(2021JCTJ0030);自治区青年拔尖人才工程(2019);宁夏大学贺兰山学者项目(2020)。

摘　　要：为了探索咖啡酸的体内代谢过程,本研究从人体肠道粪便中分离筛选出2株能代谢咖啡酸的菌株,通过细胞形态、16S rDNA序列和系统发育树分析了其菌株特征,并研究了两株菌株代谢咖啡酸过程中的细菌总数和pH变化,最后采用薄层层析法和高效液相色谱法对代谢产物进行确定,阐明咖啡酸代谢过程。结果表明,2株菌分别为腐生葡萄球菌(Staphylococcus xylosus)和奇异变形杆菌(Proteus mirabilis)。腐生葡萄球菌代谢咖啡酸含量从0.539 mg/mL在6 h后降至0.087 mg/mL,12 h后完全被代谢。而奇异变形杆菌,在6 h后咖啡酸含量从0.543 mg/mL降至0.352 mg/mL,24 h后完全被代谢。咖啡酸抑制了肠道菌的增殖,且腐生葡萄球菌代谢咖啡酸能力优于奇异变形杆菌,肠道菌代谢咖啡酸产物为间香豆酸。研究结果为进一步研究咖啡酸和肠道菌的相互作用提供依据。In order to explore the metabolic process of caffeic acid in vivo,two strains that could metabolize caffeic acid were isolated and screened from human intestinal feces,their strain characteristics were analyzed by cell morphology,16S rDNA sequence and phylogenetic tree,and the total number of bacteria and pH changes in the process of caffeic acid metabolism in the two strains were studied,and finally the metabolites were determined by thin layer chromatography and high performance liquid chromatography to elucidate the caffeic acid metabolism process.The results showed that the two strains were Staphylococcus xylosus strain and Proteus mirabilis strain.The content of caffeic acid metabolized by Staphylococcus xylosus strain decreased from 0.539 mg/mL to 0.087 mg/mL after 6 h and was completely metabolized after 12 h.However,the content of caffeic acid metabolized by Proteus mirabilis strain decreased from 0.543 mg/mL to 0.352 mg/mL after 6 h,and was completely metabolized after 24 h.Caffeic acid inhibited the proliferation of intestinal bacteria,and the ability of Staphylococcus xylosus strain to metabolize caffeic acid was better than that of Proteus mirabilis strain,and the product of caffeic acid metabolized by intestinal bacteria was m-coumaric acid.The results of the study provided a basis for further research on the interaction between caffeic acid and intestinal bacteria.

关 键 词：咖啡酸 腐生葡萄球菌 奇异变形杆菌 筛选 代谢产物 

分 类 号：R378[医药卫生—病原生物学]