Effect of Naoluoxintong formula(脑络欣通方)and its split prescriptions on cerebral vascular regeneration in rats with the cerebral ischemia-reperfusion  

作　　者：SHI Xiao WANG Lina HU Jianpeng ZHANG Limiao WANG Jin 

机构地区：[1]Graduate School,Anhui University of Chinese Medicine,Hefei 230012,China [2]Key Laboratory of Xin’an Medicine,Ministry of Education,Anhui University of Chinese Medicine,Hefei 230012,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第6期1140-1149,共10页中医杂志（英文版）

基　　金：Second Round Construction Project of state administration of Traditional Chinese Medicine Academic School Inheritance Studio[Letter of Human Teaching(2019)No.62 from State Administration of Traditional Chinese Medicine],the 13th Batch of"Innovation Team for Prevention and Treatment of Encephalopathy by Combination of Acupuncture and Medicine"Project of 115 Industry Innovation Team in Anhui Province[Anhui Talent Office(2020)No.4];Anhui Provincial Natural Science Foundation of China,studied the effects of Yiqi Huoxue Tongluo Method on Vascular Regeneration in Rats with Cerebral Ischemia and its Mechanism Based on Hypoxia-Inducible Factor 1Α/Vascular Endothelial Growth Factor Signaling Pathway(KJ2022A0547)。

摘　　要：OBJECTIVE:To observe regulatory effect of Naoluoxintong formula(脑络欣通方,NLXT)and its split prescriptions on vascular regeneration of rats suffering from cerebral ischemia-reperfusion(IR)syndrome of Qi deficiency with blood stasis(QDBS).METHODS:NLXT is the representative prescription of Yiqi Huoxue Tongluo decoction(益气活血通络方),and NLXT is divided into Yiqi herbs(益气药)and Huoxue Tongluo herbs(活血通络药)according to their efficacies.One hundred and eight specific-pathogen-free,clean-grade,Sprague-Dawley male rats were selected to prepare the classical rat model with QDBS due to middle artery ischemia-reperfusion using the multi-factor compound simulation approach.The animals were classified into sham operation(S),model(M),Nimodping(NMDP),NLXT,YQ and HXTL groups,each having 18 rats.Cerebral ischemia was reperfused after 2 h,and 24 h later,they were administered traditional Chinese medicine treatment for 14 d twice a day.Angiogenesis changes after NLXT administration to middle cerebral artery occlusion-reperfusion(MCAO/R)rats with QDBS were analyzed using the neurological deficit score and hematoxylin-eosin staining.Cerebral infarct area by 2,3,5-Triphenyltetrazolium chloride was detected,and the ultrastructure of the blood vessel in the ischemic frontoparietal cortex was observed by transmission electron microscopy.Angiopoietin 1(Ang1),angiopoietin 2(Ang2),vascular endothelial growth factor A(VEGFA),vascular endothelial growth factor receptor 2(VEGFR2),platelet endothelial cell adhesion molecule-1(CD31),angiopoietin receptor 2(Tie2),and P38 mitogen-activated protein kinase(MAPK)protein levels in the frontal and parietal cortex were quantified by immunofluorescence,reverse transcription-polymerase chain reaction,and Western blotting assays.RESULTS:Relative to the S group,VEGFA and VEGFR2 levels in the frontal and parietal cortex of group M were increased,and Ang1,Ang2,Tie2,CD31,and p38 MAPK levels remarkably increased(P<0.05);cerebral infarct area was significant and pathological morphol

关 键 词：INFARCTION middle cerebral artery REPERFUSION decomposed recipes ANGIOGENESIS Naoluoxintong 

分 类 号：R285.5[医药卫生—中药学]