食品添加剂二氧化钛和纳米二氧化钛对大鼠肝脏DNA损伤作用的评价  

Evaluation of DNA damage caused by the food additive titanium dioxide and nano-titanium dioxide

作　　者：孙拿拿 张倩男 梁春来 支媛 刘海波 方瑾 李永宁 贾旭东 SUN Nana;ZHANG Qiannan;LIANG Chunlai;ZHI Yuan;LIU Haibo;FANG Jin;LI Yongning;JIA Xudong(NHC Key Laboratory of Food Safety Assessment,China National Center for Food Safety Risk Assessment,Beijing 100021,China)

机构地区：[1]国家食品安全风险评估中心,国家卫生健康委员会食品安全风险评估重点实验室,北京100021

出　　处：《中国食品卫生杂志》2023年第9期1258-1264,共7页Chinese Journal of Food Hygiene

基　　金：国家食品安全风险评估中心高层次人才团队建设项目;国家重点研发计划(2019YFC1605203)。

摘　　要：目的利用哺乳动物体内碱性彗星试验,评价食品添加剂二氧化钛和纳米二氧化钛对大鼠肝脏细胞DNA的损伤作用。方法依据哺乳动物体内碱性彗星试验指南(OECD TG489),将雄性SD大鼠随机分成9组,每组5只。食品添加剂二氧化钛设1000、500、250 mg/kg·BW 3个剂量组,阴性对照为纯水;纳米二氧化钛设500、150、50 mg/kg·BW 3个剂量组,阴性对照为0.8%吐温+3%FBS。通过灌胃方式给予,每日1次,共15 d;同时设置阳性对照组,阳性物甲基磺酸乙酯(EMS)仅在第14、15天每日灌胃一次给予,剂量为200 mg/kg·BW·d。末次灌胃6 h后,麻醉条件下取大鼠肝脏,制备成单细胞悬液后涂片,经裂解、解旋、电泳、染色等步骤,进行彗星图像分析。结果食品添加剂二氧化钛和纳米二氧化钛各剂量组大鼠肝脏细胞的尾部DNA含量百分比与相应阴性对照组相比均无显著性差异(P>0.05),而EMS组大鼠肝脏细胞的尾部DNA含量百分比与阴性对照组比较均具有显著差异(P<0.01)。结论在本实验条件下,食品添加剂二氧化钛和纳米二氧化钛均未诱导大鼠肝脏细胞的DNA损伤。Objective Applying the in vivo Mammalian Alkaline Comet Test,this study aimed to investigate the DNA damage caused by the food additive titanium dioxide and nano-titanium dioxide on rat liver cells.Methods According to the guideline“In vivo mammalian alkaline comet assay”(OECD TG489),male SD rats were randomly divided into nine groups,five rats per group.Food additive titanium dioxide was administered to three groups at doses of 1000,500 and 250 mg/kg·BW,while pure water served as the negative control.Titanium dioxide nanoparticle was administered in three different doses:500,150,and 50 mg/kg·BW.The negative control group received 0.8%Tween+3%FBS.Test substances were administered by gavage once a day for 15 d.Simultaneously,a positive control group was established,and the positive substance ethyl methyl sulfonate(EMS)was administered once daily on the 14th and 15th days via gavage,at a dosage of 200 mg/kg·BW·d.Animals were anesthetized six hours after the last administration,and the livers were harvested to prepare single-cell suspension smears.The sections were subsequently subjected to lysis,unwinding,electrophoresis,staining to acquire the comet images for analyzing.Results There were no statistical differences(P>0.05)in the percentages of tail DNA content between the treated and negative control rats.However,the percentage of tail DNA content of rat liver cells in the EMS group showed a significant difference compared to the negative control group(P<0.01).Conclusion Under the circumstances of our test,neither the food additive titanium dioxide nor nano-titanium dioxide caused the DNA damage in rat liver cells.

关 键 词：食品添加剂 二氧化钛 纳米二氧化钛 DNA损伤 大鼠肝脏细胞 

分 类 号：R155[医药卫生—营养与食品卫生学]