Coumarin and eugenol ameliorate LPS-induced inflammation in RAW 264.7 cells via modulating the NLRP3 inflammasome pathway  

作　　者：Jyotsana Bakshi Somnath Singh KP Mishra 

机构地区：[1]DRDO Defence Institute of Physiology and Allied Sciences,Delhi,India

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2024年第1期40-46,共7页亚太热带生物医学杂志（英文版）

基　　金：supported by the Defence Institute of Physiology and Allied Sciences.

摘　　要：Objective:To investigate the underlying mechanism of anti-inflammatory action of coumarin and eugenol in lipopolysaccharide(LPS)-stimulated RAW 264.7 cells.Methods:RAW 264.7 cells were treated with 2.5μg/mL of LPS,50μM of coumarin,and 50μM eugenol for 24 h.The viability of the cells was assessed using MTT assay.The production of nitric oxide was determined using Griess reagent and DCFH-DA was used to measure the production of reactive oxygen species.The protein expression of NLRP3,IL-1β,NF-κB,and cyclooxygenase 2 was assessed using Western blot analysis.Results:Coumarin and eugenol showed anti-inflammatory effects against LPS-induced inflammatory response by ameliorating the expression of NLRP3 inflammasome and NF-κB,which further led to a subsequent reduction in IL-1β,nitric oxide,and reactive oxygen species.Conclusions:Coumarin and eugenol exert their anti-inflammatory activities by modulating the NLRP3 inflammasome pathway and NF-κB.These compounds may have promising therapeutic applications for the treatment of various inflammatory diseases.

关 键 词：INFLAMMATION CYTOKINES COUMARIN EUGENOL NLRP3 LPS 

分 类 号：R285[医药卫生—中药学]