花青素(C3G)通过抗氧化抑制自噬缓解大鼠痫样发作  

Anthocyanins (C3G) alleviate epileptic seizures throughantioxidant inhibition autophagy in the rat

作　　者：王梓 宁瑞 张翔威 吴淑华[2] 郭科[1] 郭翀 李建民[1] WANG Zi;NING Rui;ZHANG Xiangwei;WU Shuhua;GUO Ke;GUO Chong;LI Jianmin(Department of Neurosurgery,Affiliated Hospital of Binzhou Medical University,Binzhou 256603,China;Department of Pathology,Affiliated Hospital of Binzhou Medical University,Binzhou 256603,China)

机构地区：[1]滨州医学院附属医院神经外科,滨州256603 [2]滨州医学院附属医院病理科,滨州256603

出　　处：《神经解剖学杂志》2023年第6期696-704,共9页Chinese Journal of Neuroanatomy

基　　金：山东省医药卫生科技发展计划(2017WS553)。

摘　　要：目的:研究花青素(C3G)通过抗氧化抑制自噬缓解癫痫的可行性。方法:SD大鼠75只随机分为对照组、戊四氮(PTZ)组、过氧化氢(H_(2)O_(2))干预组、3-甲基腺嘌呤(3-MA)干预组和花青素(C3G)干预组。记录各组大鼠癫痫的发作等级、潜伏期及次数,脑电图检测大鼠的脑异常放电情况,膜片钳检测大鼠海马神经元动作电位,试剂盒检验大鼠海马区4-羟基壬烯酸(4-HNE)的含量,电镜观察大鼠海马区神经元超微结构变化;尼氏染色检测大鼠海马区神经元损伤情况;免疫组织化学染色和Western Blot检测各组大鼠海马脂氧合酶15(15-LOX)和谷胱甘肽过氧化酶4(GPX4)的表达,以及微管相关蛋白1轻链3(LC3)亚型的比例变化。结果:与对照组比较,PTZ组大鼠完全点燃,建模成功;C3G组与其他癫痫组比较,癫痫发作级别下降,异常放电减少,潜伏期变长,海马神经元兴奋性降低,尼氏体含量增加,4-HNE含量、15-LOX表达量和LC3II/LC3I比值均降低,GPX4表达升高(P<0.05)。结论:癫痫引发的氧化应激可诱导神经元过度自噬,花青素可以通过抗氧化并抑制自噬从而缓解癫痫的发生发展。Objective:To investigate the feasibility of anthocyanins(C3G)antioxidant inhibition of autophagy to alleviate epilepsy.Methods:Seventy-five SD rats were randomly divided into 5 groups:Control group,pentetrazole(PTZ)group,hydrogen peroxide(H_(2)O_(2))intervention group,3-methyladenine(3-MA)intervention group,and C3G intervention group.The seizure grade,latency,and frequency were documented.Electroencephalography was employed to detect abnormal electrical discharges in the brain across.Patch clamp technique was utilized to measure action potentials in hippocampal neurons for each group.The concentration of 4-hydroxynonenoic(4-HNE)hippocampus was determined using a specific kit.Ultrastructural alterations in hippocampal neurons were examined through electron microscopyissl staining was performed to assess neuronal damage within the hippocampus.Immunohistochemical staining and Western Blot were conducted to evaluate expression levels of 15-LOX,GPX4,and LC3 proteins within the hippocampus of rats.Results:Compared with the control group,the PTZ group was completely ignited and the modeling was successful.Compared with other epilepsy groups,the seizure grade of C3G group decreased,abnormal discharge decreased,latency increased,hippocampal neuron excitability decreased,nishi content increased,4-HNE content,15-LOX expression and LC3II/LC3I ratio decreased,but GPX4 expression increased(P<0.05).Conclusion:The oxidative stress induced by epilepsy can induce excessive autophagy of neurons,and C3G can alleviate the occurrence and development of epilepsy by anti-oxidation and inhibition of autophagy.

关 键 词：癫痫 花青素 氧化应激 自噬 动作电位 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]