房式仓储藏对进口大豆真菌群落多样性的影响  

Effects of House Warehouse Storage on Fungal Community Diversity of Imported Soybean

作　　者：李欣蔚 李佳 林琳 陈怡岑 曹毅 赵旭 LI Xin-wei;LI Jia;LIN Lin;CHEN Yi-cen;CAO Yi;ZHAO Xu(Liao Ning Grain Science Research Institute,Shenyang,Liaoning 110032,China)

机构地区：[1]辽宁省农科院粮食科学研究所,辽宁沈阳110032

出　　处：《粮油食品科技》2024年第1期139-145,共7页Science and Technology of Cereals,Oils and Foods

基　　金：辽宁省科学计划项目面上项目(2023010714-JH3/107)~~。

摘　　要：为探究房式仓储藏对进口高油大豆真菌群落多样性的影响,以当年收获的高油大豆为实验材料在房式仓中过夏储藏3个月后,定期取样测定水分,并利用IlluminaMiseq高通量测序技术分析原始状态和其他储藏阶段大豆中真菌群落状态。结果表明,不同储藏时期的大豆样品中的真菌群落共计属于4个门,9个纲,14个目,15个科,21个属,19个种。大豆在不同储藏时期的4个优势菌门为子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、羊肚菌门(Mortierellomyco)、毛霉菌门(Mucoromycota)。在种的水平上进行分类统计分析发现,相对丰度较高的10个菌种在数量上均呈现随着储藏时间的延长而有不同程度增加的趋势(C47>B47>A47),并发现了1种有害真菌赤霉菌。通过Ven图分析和Beta多样性分析发现,在房式仓中储藏的前两个储藏期的大豆样品中真菌群落结构具有较高的相似度,而到了第三储藏期与前两者真菌群落结构差异较大,说明到了第三储藏期大豆中的真菌群落不仅多样性显著增加,结构也发生了明显演替。In order to explore the effects of house warehouse storage on fungal community diversity of imported soybean,Illumina MiSeq high-throughput sequencing was employed to analyze the fungal community structure and diversity of newly harvested imported high oil soybean for 3 months with periodic sampling for moisture determination.The result demonstrated that the fungal communities in different storage periods in the high oil soybean belonged to 4 phyla,9 classes,14 orders,15 families,21 genera and 19 species in accordance with the 97%sequence similarity level.The four dominant phyla of soybean in different storage periods were Ascomycota,Basidiomycota,Mortierellomyco and Mucoromycota.At the species level, classification statistical analysis showed that the 10 strains with relatively high abundance presented an increasing trend with the extension of storage time (C47>B47>A47). In addition, quarantine pathogenic fungus gibberella were found. Through the analysis of Ven map and Beta diversity, it was found that the fungal community structure of soybean samples in the first two storage periods was relatively similar. The fungal community structure of soybean samples in the third storage period was significantly different from that in the first two storage periods with the storage of house warehouse. It could be concluded that the fungal community diversity of soybean samples in the third storage period not only increased significantly, but also experienced obvious succession of fungal community structure.

关 键 词：进口大豆 高通量测序 真菌群落结构 安全储藏 

分 类 号：TS201.3[轻工技术与工程—食品科学] S-3[轻工技术与工程—食品科学与工程]